复方扶芳藤合剂服结合BMSCs配合FG移植对兔膝关节软骨组织形态学的影响.pdfVIP

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复方扶芳藤合剂服结合BMSCs配合FG移植对兔膝关节软骨组织形态学的影响.pdf

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复方扶芳藤合剂服结合BMSCs配合FG移植对兔膝关节软骨组织形态学的影响

目录中文摘要 ···················································1 英文摘要 ···················································3 引言 ····················································7 正文 ····················································9 第一部分 ···················································9 1.实验材料和方法 ·········································9 1.1实验动物··················································9 1.2主要试剂 ··················································9 1.3 主要仪器 ················································10 1.4 主要试剂的配制 ··········································11 1.5 实验方法···············································12 2. 实验结果 ···············································13 2.1 BMSCs 形态学观察 ········································13 2.2 BMSCs 复苏后生长情况 ····································14 2.3 成骨细胞、成脂肪细胞诱导分化的鉴定 ·····················14 3.讨论 ················································14 3.1BMSCs 在组织工程中的作用及意义 ···························14 3.2BMSCs 的分离方法及意义 ··································15 3.3 BMSCs 的形态与体外生长特点 ······························16 3.4 BMSCs 的鉴定············································17 3.5 BMSCs 的冻存与复苏 ·····································17 4.存在的问题和展望 ······································18 第二部分 ··················································20 1 材料与方法 ················································20 1.1 材料 ····················································20 I 1.2 动物模型的制备 ··········································21 1.3 实验分组及喂养·········································21 1.4 BMSCs+FG 复合物制备 ····································21 1.5 治疗方法 ···············································22 1.6 取材及病理切片 ··········································22 1.7 染色方法 ···············································22 1.8 观察指标 ···············································24 1.9 统计学方法 ······························