四川大头茶在不同群落中的遗传分化及适合度成分.pdfVIP

第25卷第 1期 生 态 学 报 Vol.25,No.1 2005年 1月 ACTA ECOLOGICA SINICA Jan.，2005 四川大头茶在不同群落中的遗传分化及适合度成分 操国兴,2‘,3，钟章成2 谢德体3，刘 芸3 (1.四川农业大学林学园艺学院，雅安 四川 625014;2.西南师范大学生命科学学院，重庆 400716; 3.西南农业大学资源环境学院，重庆 400716) 摘要:研究了四川大头茶在3个群落中的遗传分化和适合度成分。12个引物的RAPD分析表明，仅有10%左右的遗传多样性 存在于种群间。四川大头茶纯林结实率(31.86%)显著高于针阔混交林(22.53%)和常绿阔叶林(24.52%)。每果种子数和每果 种子均重各种群间差异均显著，每果种子数和每果种子均重分别为常绿阔叶林(29.50,0.0154g)，四川大头茶纯林(28.39, 0.0172g)，针阔混交林(27-44,0.0195g)，表明二者间存在着负祸联关系(tradeoff)。而以R一艺hb二表示的适合度却以常绿阔 叶林最大。 关键词:四川大头茶;群落;遗传分化;适合度 GeneticvariationandreproductivefitnesscomponentsofGordoniaacuminatain differentcommunities CAOGuo-Xings，ZHONGZhang-Cheng，XIEDe-Ti3，LIUYun3 (1.ForestryandHorticutureCollege,Sichuan AgriculturalUniversity.YaanSichuan625014,China;2.FacultyofLifeScience,SouthwestNormalUniversity,Chongqing400715,China;3. FacultyofResourceandEnvironments,SouthwestAgriculturalUniversity,Chongqing400716,China).ActaEcologicaSinica,2005,25(1):13- 17. Abstract:Gordoniaacuminataisoneofthedominanttreespeciesofevergreenbroadleveadforestanditoccursinsuccession seriescommunitiesinMt.Jinyun.Threepopulationsatdifferentsuccessionalstages，i.e,mixedconiferousevergreen broadleavedforest(MF)，Gordoniaacuminataforest(GF)andevergreenbroadleavedforest(EF)，andclosetoeachotherat geographicdistancewereselectedtoevaluatetheirgeneticdiversityandreproductivefitness. 12sampleswerecollectedalongalongdiagonaltransectineachpopulation.RAPD (Randomamplifiedpolymorphic DNA)wasusedtodetectgeneticvariationwithinandamongpopulations.Atotalof40randomprimerswereinitiallyscreened using2representativesfromeachofthethreepopulations.12primersthatgavedistinct,repeatablefragmentpatternswere selectedforfin