姜黄素单羰基类似物的体外稳定性及其抗肿瘤活性研究.doc

姜黄素单羰基类似物的体外稳定性及其抗肿瘤活性研究 杨苹1a 隋思博2 张敏1b梁广3王艳芳1a 官丽莉1a 李校堃1.3* （1.吉林农业大学 a. 教育部生物反应器与药物开发工程研究中心 b. 农业部参茸质量监督检验测试中心 长春 130118；2.吉林省食品药品检验所 长春 130033； 3.温州医学院药学院 温州325035; ) 摘要 目的：研究姜黄素及合成的姜黄素单羰基类似物在体外的稳定性及其抗肿瘤活性。方法：将姜黄素及合成化合物加入到配制的溶液中，计算其在溶液中的降解率，并以此为指标，考察其在体外的稳定性；同时用MTT法分析其对肿瘤细胞生长的影响；结果：在相同条件下，姜黄素及其类似物的稳定性依次为：B类≥C类≥A类≥姜黄素，抗肿瘤活性依次为：B类≥C类≥A类≥姜黄素；结论：合成的姜黄素类似物不仅提高了体外的稳定性，且其抗肿瘤活性也优于姜黄素。 关键词：姜黄素 单羰基类似物 稳定性 抗肿瘤活性 [中图分类号] [文献标识码] [文章编号] Research on the Stability and Anti-tumor Propertie of Mono-carbonyl Analogues of Curcumin Yang Ping1a Sui Si-bo2 Zhang Min1b Wang Yan-fang1a Guan Li-li1a Liang Guang3 Li Xiao-kun1,3* (1. Jilin Agricultural University，a. Engineering Research Center of Bioreactor and Pharmaceutical Development，Ministry of Education. b. Genseng and antler products centre of the ministry of agriculture Changchun 130118；2.Jilin Institute of Food and Drug Control，Changchun 130033；3. Schoo Pharmacy, Wenzhou Medical College , Wenzhou 325035) Abstract Objectives: To research the stability and anti-tumor propertie of mono-carbonyl analogues of Curcumin in vitro. Methods: Curcumin and synthetic compounds were added the preparation of the solution，the degradation rate of solution was used to evaluate the stability in vitro. At the same time using the MTT method analyzed the inhibitory anti-tumor propertie effect [基金项目] 吉林农业大学青年基金（No.201127） [作者简介]杨苹，女，硕士研究生，研究方向：中药新药资源开发与利用，TelE-mail：yp-yy-113@163.com [通讯作者] 李校堃，男，博士学位，教授，研究方向：中药新药资源开发与利用； Tel：0431E-mail：xiaokunli@163.net of mono-carbonyl analogues of Curcumin on tumor cell line. Results: Under the same conditions, the stability of Curcumin and Curcumin derivatives by compound B is best, followed by compound C and then compound A,and the anti-tumor propertie as well; Conclusions: The mono-carbonyl analogues of Curcumin improve both the stability of the in vitro and the anti-tumor propertie compared with Curcumin . Key words: Curcumin；Mono-carbonyl analogues；Stability；Anti-tumor propertie；