- 1、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。。
- 2、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 4、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 5、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
非洲猪瘟1
* Dr Corrie Brown University of Georgia * corrie * #15 ASF (PIADC) Engorged spleen * #16usda ASF Paracortical hemorrhage in gastrohepatic lymph node. * USDA PIADC * piadc * USDA PIADC * USDA PIADC * Mvv internal bleeding all organs; cortx of kidney Bowel image: piadc * MVV Blood clots in lymphatics and mesenteric ln * MVV Enlargement of spleen in most cases, not always. Hemorrhagic GI * * MVV hydropericarditis, lung edema * DPI Days Post-Infection * USDA PIADC * USDA PIADC * Images from Dr Corrie Brown * Taken from DR McKenna’s presentation from Colo. FEAD course Aug 1-5, 2005 猪发热的时候就要怀疑非洲猪瘟 ASF should always be suspected where there are febrile pigs. 坏死包括 Necropsy findings include: 脾 Greatly enlarged spleen, dark red to black in color, friable spleen 肝肠淋巴结 Very enlarged, hemorrhagic gastrohepatic lymph nodes 肾淋巴结Very enlarged, hemorrhagic renal lymph nodes African Swine Fever 2013 Diagnosis Hog Cholera vs. African Swine Fever 猪瘟引起消瘦而非洲猪瘟不引起体重变化 HC infected pigs become depressed and lose weight, whereas ASF infected pigs have neither symptoms 猪瘟引起恶臭的腹泻 HC is also characterized by a foul-smelling diarrhea African Swine Fever 2013 Diagnosis Laboratory Specimens 血清Serum / clotted blood 抗凝血EDTA, heparin blood 淋巴结Lymph nodes 脾Spleen 扁桃体Tonsil 肺Lung 肝Liver 肾Kidney African Swine Fever 2013 Laboratory Diagnosis 病毒分离 Virus isolation - Haemadsorption test (HAD) of leukocyte cultures.- Haemadsorption autorosette test of PBLs of suspect pigs. 接种猪 Pig inoculation - Requires inoculation of na?ve and CSF-vaccinated pigs.- Not recommended with newer tests available. African Swine Fever 2013 Laboratory Diagnosis 病毒抗原检测(DFAT) Virus antigen detection- Direct fluorescent antibody test (DFAT) 病毒基因检测(PCR) Virus genome detection- Polymerase Chain Reaction (PCR) - African Swine Fever 2013 Bastos, Penrith, Cruciere, et al. Arch Virol. 2003 148(4):693-706. Genotyping field strains of African swine fever virus by partial p72 gene charac
文档评论(0)