适用于不同尺寸血管的脱细胞方法的研究.pdfVIP

4 ( ) No. 4 2012 7 Journal of East China Normal University (Natural Science) Jul. 2012 Article ID: 1000-5641(2012)04-0050-11 A general decellularization method for preparing vascular scaffolds from blood vessels of different types, diameters and wall-thicknesses 1 2 1 ZHANG Hong-xia , ZHAI Wan-yin , ZHANG Hong-feng (1. School of Life Science, East China Normal University, Shanghai 200062, China; 2. State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China) Abstract: This study was to build a general decellularization method by combining trypsinization with repeated frozen/thawing treatment for different types of vessels includ- ing saphenous vein, carotid artery and aorta. Saphenous vein, carotid artery and aorta were decellularized by trypsinization and repeated frozen/thawing treatment. The efficiencies of cell removal and extracellular matrix (ECM) integrity were examined by Hematoxylin and eosin staining, Masson Trichrome staining and Weigert’s staining. A quantitative means based on image analysis software was used to quantify the ECM preservation. Scanning electron microscopy was used to show the extracellular matrix integrity. Histology staining and quantitative analysis demonstrated trypsinization with repeated frozen/thawing treat- ment decellularized the saphenous vein, carotid artery and aorta completely. The ECM structure was optimally preserved and integrity. Scanning elec