影响双向电泳分离效果的若干实验条件比较研究.pdfVIP

33 5 () Vol.33 No.5 2005 10 Journal of Fuzhou niversity(Natural Science) Oct. 2005 : 1000- 2243(2005)05- 0674- 05 1 1 1 2 2 1 , , , , , ( 1. , 350002; 2. ATTO , 113- 8425) : pH ( ISO- DALT) . , ; , ; ; 5 10 Da . : ; ; : Q5- 33; Q510. 2 : A Effect of experimental conditions on the resolution of the twodimensional electrophoresis 1 1 1 2 2 1 CHEN Hua , LI Shu- tao , WEN Teng , HARAYAMA Takeshi , K BOTA Hidehiro , RAO Ping- fan (1. Institute of Biotechnology, Fuzhou niversity, Fuzhou, Fujian 350002, China; 2. The Department of RD, ATTO Corporation, Tokyo 113- 8425, Japan) Abstract: The effect of several experimental conditions on the result of ISO- DALT was investigated in this paper. It was found that the purity of agarose and urea dramatically influenced the separation, the addition of acrylamide or iodoacetamide into the sample reduced by DTT could decrease the pseudo dots of protein, thinner separating gel exhibited better effect, thereplacement of agarosefor polyacrylamide as 5 carrier of amphelyte could separate protein with molecular weight more than 10 Da. Keywords: two- dimensional electrophoresis; carrier amphelyte; experimental condition , pH pH . , pH [ 1] , . pH , pH 5 , , 10 Da . , pH (ISO- DALT) . [2] , Na2S2O3 , , , . Na2S2O3 , . ( ) . , , , . 1 1.1 ,