深沟球轴承PROE绘制课程.docVIP

深沟球轴承 说明:按照GB/T4459.7-1998《机械制图 滚动轴承表示法》及GB/T276-1994的给出的近似画法;要精确绘制需要查询有关轴承的精确数值?本例的轴承代码是6308,内径为40,外径为90,宽为23?由于深沟球轴承是标准件,只需绘出外形尺寸,保证安装尺寸即可,没必要精确其具体数值?如果就是要进行深沟球轴承的精确建模,则需要查标准确定准确数值? 1.在“工具→参数”中添加参数; 轴承内径 dx 40 轴承外径 da 90 轴承宽度 b 23 2.front面上旋转,绘制下图的草绘,尺寸计算可见《机械制图》236页,完成后旋转成实体,退出旋转,添加关系式; d2=b d3=da d4=dx d1=60 d5=(da-dx)/8 3.front面上旋转,添加必要的草绘参照,绘制下图的草绘(为长方形),完成后旋转成实体,此部分为轴承的保持架; 4. 偏移Right面创建基准平面DTM1,退出后添加关系式d8=(da+dx)/4; 5.front面上旋转,绘制保持架中的缺口; 6.进行圆周阵列,个数为7; 7.front面上旋转,绘制滚珠; 8.进行圆周阵列,个数为7; 9.进行内外圈进行倒角处理C1; Acknowledgments The authors would like to thank Johns Hopkins University for the TC-1 cells. This work was supported by a National Health Research Institutes intramural grant (IV-103-PP-22) and grants from the National Science Council, which were awarded to Y.C. Song (NSC 99-2321-B-400-004-MY3) and S.J. Liu (NSC 103-2321-B-400-008). Author Contributions Y.C.S. and S.J.L. designed the studies. Y.C.S. performed the research and analyzed the data. Y.C.S. and S.J.L. wrote the manuscript. Additional Information C57BL/6 mice were immunized subcutaneously (s.c.) once with 1 μ g of peptide mixed with or without 10 μ g of CpG adjuvant. After one week, splenocytes were harvested, and the response of IFN-γ -secreting cells was determined by ELISPOT after 48 h of peptide stimulation. Briefly, 2 × 105 splenocytes were incubated with 1 μ g/ml irrelevant peptide or RAH peptide in an anti-IFN-γ -coated polyvinylidene fluoride (PVDF) plate for 48 h. After incubation, the cells were removed, and a biotinylated anti-IFN-γ Ab (eBioscience, San Diego, CA, USA) was added to each well. The plates were incubated at 37 °C for 2 h. Following the addition of the avidin-HRP reagent (eBioscience, CA, USA), the assay was developed using a 3-amine-9-ethyl carbazole (AEC; Sigma-Aldrich, MO, USA) staining solution. The reaction was stopped after 4–6 min by placing the plate under tap water. The spots were counted using an ELISPOT reader (Cellular Technology Ltd., Shaker Heights, OH,