水闸工作桥内力计算方法课程.docVIP

水闸工作桥的内力计算 ????一????水闸属于既能关闭闸门拦挡水流,又能开启闸门泄(引)水的建筑物工作桥是为了安置闸门启闭设备和工作人员操作而设置的,利用两根预制T型或Ⅱ型梁进行现场吊装拼接而成 ? ????二T形梁的内力计算????如图所示为一三孔拦洪闸的示意图,选用2 157KN双吊点卷扬式起闭机,机座重9.81KNT形梁上,试设计一钢筋混凝土T形梁已知条件:悬臂板自重:q1 =3.43KN/m????????梁自重: ????q2 =3.68 KN/m????????栏杆重: ????q3 =1.47 KN/m????????人群荷载: ??q4= 4.86 KN/m????????梁上均布荷载:q = q1 + q2 +q3+q4 =13.14 KN/m????????启门力(每一根梁各负担一半)P = (157+9.81)/2=83.41KNT形梁为搁置在工作桥墩上的简支梁?梁净跨 ,?计算跨度??????????取l=6.72m荷载至支座中心距离 ? ????计算步骤:????(1)支座反力计算????支座反力 ????(2)内力计算:????弯矩计算: ???????? ????剪力计算: 支座边缘 ????????????(3)绘制梁的简力图和弯矩图,如图(b)(c)所示 ? Acknowledgments The authors would like to thank Johns Hopkins University for the TC-1 cells. This work was supported by a National Health Research Institutes intramural grant (IV-103-PP-22) and grants from the National Science Council, which were awarded to Y.C. Song (NSC 99-2321-B-400-004-MY3) and S.J. Liu (NSC 103-2321-B-400-008). Author Contributions Y.C.S. and S.J.L. designed the studies. Y.C.S. performed the research and analyzed the data. Y.C.S. and S.J.L. wrote the manuscript. Additional Information C57BL/6 mice were immunized subcutaneously (s.c.) once with 1 μ g of peptide mixed with or without 10 μ g of CpG adjuvant. After one week, splenocytes were harvested, and the response of IFN-γ -secreting cells was determined by ELISPOT after 48 h of peptide stimulation. Briefly, 2 × 105 splenocytes were incubated with 1 μ g/ml irrelevant peptide or RAH peptide in an anti-IFN-γ -coated polyvinylidene fluoride (PVDF) plate for 48 h. After incubation, the cells were removed, and a biotinylated anti-IFN-γ Ab (eBioscience, San Diego, CA, USA) was added to each well. The plates were incubated at 37 °C for 2 h. Following the addition of the avidin-HRP reagent (eBioscience, CA, USA), the assay was developed using a 3-amine-9-ethyl carbazole (AEC; Sigma-Aldrich, MO, USA) staining solution. The reaction was stopped after 4–6 min by placing the plate under tap water. The spots were counted using an ELISP