一种复合菌制剂对富营养化污水的净化作用研究.pdfVIP

第 32 卷第9 期 环　 境　 科　 学　 学　 报 Vol.32,No.9 2012年9月 　Acta Scientiae Circumstantiae Sep. ,2012 杨小龙,李文明,曹郁生.2012.一种复合菌制剂对富营养化污水的净化作用研究[J].环境科学学报,32(9):2071-2076 Yang X L,Li WM,CaoYS. 2012.Purificationeffectof acompound-bacteriaagentoneutrophicwastewater[J].ActaScientiaeCircumstantiae,32(9): 2071-2076 一种复合菌制剂对富营养化污水的净化作用研究 杨小龙,李文明,曹郁生∗ 食品科学与技术国家重点实验室,南昌大学中德联合研究院,南昌330047 收稿日期:2011-10-21　 　 　 修回日期:2011-12-14　 　 　 录用日期:2011-12-31 摘要:以高效去除COD为主要指标,从南昌市区富营养化的鱼塘淤泥水和猪场废水混合液中分离筛选出4 株目的菌株,发现其中以NCG1菌 株的去除活性最好.经生理生化鉴定和16SrRNA序列分析,初步鉴定NCG1为施氏假单胞菌(Pseudomonasstutzeri).同时,采用另一株具有高效 去除无机盐氮能力的菌株C-4 与菌株NCG1复合培养,建立了两株菌的复合培养方法.结果表明,与单菌相比,复合菌综合了单菌的优良去除 + - 活性,在对合成废水的处理中,72 h后废水中NH -N、NO -N和COD的去除率分别达84.4%、86.91%和81.36%.研究表明,生产的复合菌剂 4 3 在对富营养化池塘污水样品的处理中显示出良好的效能. 关键词:复合菌制剂;污水净化;COD去除 文章编号:0253-2468(2012)09-2071-06　 　 　 中图分类号:X703.1　 　 　 文献标识码:A Purification effect of a compound-bacteria agent on eutrophic wastewater YANG Xiaolong,LI Wenming,CAO Yusheng∗ State Key Laboratory of Food Science and Technology,Sino-German Joint Research Institute,Nanchang University,Nanchang330047 Received21 October2011;　 　 　 received in revised form 14 December2011;　 　 　 accepted31December2011 Abstract:Targeting oneffectivelyremovingchemicaloxygendemand (COD),fourbacterialstrainswithCODremovalabilitywereisolatedfromeutrophic pond water and piggery wastewater. The isolated NCG1,identified as Pseudomonas stutzeri by morphological and physiological characteristics and 16S rRNA sequential analysis,showed the highest COD removal efficiency in the four bacteria. Synergistic effect in purification was found when NCG1 was used togetherwith C-4,astrainwithhighinorganicsaltnitrogenremovingability. Themixedtwostrainsshowedhigherabilitythansinglemicroorganism, and removed the ammonium, nitrite