低温低碳氮比好氧反硝化菌的筛选及其鉴定.pdfVIP

科技导报2014，32（11） 低温低碳氮比好氧反硝化菌的筛选低温低碳氮比好氧反硝化菌的筛选 及鉴定及鉴定 1,2 3 3 3 1 周兰影 ，马秀兰 ，张晨东 ，王呈玉 ，张凤君 1. 吉林大学地下水资源与环境教育部重点实验室，长春130021 2. 吉林省产品质量监督检验院，长春130022 3. 吉林农业大学资源与环境学院，长春130118 摘要摘要 传统的反硝化工艺存在反硝化细菌的世代时间较长（特别是在低温的冬季）、水力停留时间长、运行和投资费用大等问 题。为寻找更好的反硝化细菌，从冬季北方城市污水厂驯化活性污泥中分离出1株耐低温、低碳氮比，且脱氮率较高的好氧反硝 - 化菌株，命名为HFX08。初步鉴定该菌株为假单胞菌属（Pseudomonas），G菌。该菌株的最适生长温度为10~20℃，生长曲线 符合S型曲线，拟合方程相关性系数达到了差异极显著水平。随着碳氮比的增加，该菌株的反硝化速率随之提高，脱氮率最高可 达92%。 关键词关键词 好氧反硝化菌；假单胞菌属；脱氮率；碳氮比；低温 中图分类号中图分类号 X172 文献标志码文献标志码 A doidoi 10.3981/j.issn.1000-7857.2014.11.004 ScreeningandIdentificationofanAerobicDenitrifying BacteriumwithLowC/NRatioatLowTemperature 1,2 3 3 3 1 ZHOULanying ,MAXiulan,ZHANGChendong,WANGChengyu,ZHANGFengjun 1.KeyLaborotaryofGroundwaterResourcesandEnvironmentofMinistryofEducation,JilinUniversity, Changchun 130021,China 2.InstituteofSuperuisionInspectiononProductQualityofJilinProvince,Changchun 130022,China 3.CollegeofResourcesandEnvironment,JilinAgriculturalUniversity,Changchun 130118,China AbstractAbstract Theconventionaldenitrificationprocesshaslimitedapplicationsduetoitsdisadvantages,suchaslonggenerationtimeof denitrifyingbacteria,especiallyincoldwinter,longhydraulicretentiontimeandhighcosts.Inthisstudy,anaerobicdenitrifying bacteriumdesignatedasHFX08 wasisolatedfromadomesticsewageplantinanortherncityofChinainwinter.ThestrainHFX08 exhibitedhighdenitrificationperformanceunderlowC/Nratioatlowtemperature.TheresultsindicatedthatthestrainHFX08 wasG- andidentifiedasPseudomonas sp.Theoptimumtemperatureforitsgrowthisintherangeof 10-20℃.Itsgrowthcurvewasfittedto S growth curve, whose fitting coefficient reached the level of extremely significant difference. The nitrogen removal rate by strain HFX08 increased