肺病理取材定量比较的研究及诊断学意义.pdf

201331 383 [] 15SD 3HE (P 0.05) (P 0.05) [] []R36-33 []A []0577-7402(2013)03-0180-05 LI Hai-sheng, DU Juan, WANG Hai-yan, YUE Cai-li, YANG Ce, JIANG Jian-xin State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China This study was supported by the National Basic Research and Development Program of China (973 Program) (2012CB518104) [Abstract]ObjectiveTo qualitatively and quantitatively compare the effect of tracheal inflation method, vascular perfusion method and combined trachea-vascular perfusion method on alveolar structure and cell antigen preservation. Methods Fifteen healthy Sprague Dawley rats were randomly divided into three groups (5 each): tracheal inflation group, vascular perfusion group and combined trachea-vascular perfusion group. After lungs were respectively fixed with the methods mentioned above, tissue blocks were paraffin embedded and sectioned. HE staining and immunofluorescence staining were then performed for qualitative observation and stereological analysis of the fixation effect. ResultsQualitative study showed that the fixation effect of cytoplasm and nuclear antigen was better in vascular perfusion group and combined trachea-vascular perfusion group than in tracheal inflation group. Stereological analysis showed that the alveolar linear intercept in combined trachea-vascular perfusion group was longer than that in tracheal inflation and vascular perfusion group (P 0.05). The lung parenchyma volume density and alveolar septum thickness of combined trachea-vascular perfusion group were smallest among all the three groups, followed by tracheal inflation group and vascular pe