松江鲈鱼体表黏液抑菌效果的研究.pdfVIP

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN 年第 期 2014 9 松江鲈鱼体表黏液抑菌效果研究 邱进  潘连德  张帅 （上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室，上海 201306） 摘 要 ： 以松江鲈鱼（ ）体表黏液作为试验材料，分别用水、酸、有机物3 种不同的提取剂对其进行粗提取， Trachidermus fasciatus 采用滤纸片法和二倍稀释法确定其抑菌效果，只有酸的粗提物对所检测的6 种病原菌有明显的抑制作用，且抑制作用由大到小的 排列顺序是 ：维氏气单胞菌、表皮葡萄球菌、腐生葡萄球菌、嗜水气单胞菌、弗氏柠檬酸杆菌、沃氏葡萄球菌。用SDS定 量分析酸的粗提物蛋白质表达谱，其蛋白大小＜ 95 kD，且在34-55 kD 之间含量最高。初步研究表明松江鲈鱼体表黏液酸的粗提 物在抵抗上述病原菌入侵方面发挥了主要作用，其抗菌蛋白的确定还有待进一步分析。 关键词 ： 松江鲈鱼 体表黏液 抑菌效果 抗菌蛋白 Preliminary Investigation on Antimicrobial of the Epidermal Mucus （ ） Secretion of Roughskin Sculpin Trachidermus fasciatus Qiu Jin Pan Liande Zhang Shuai （Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources of China Ministry of Education ，Shanghai Ocean University ， Shanghai 201306 ） Abstract: The epidermal mucus samples from roughskin sculpin（Trachidermus fasciatus ）were extracted with aqueous, acidic and organic solvents to identify potential antimicrobial acitivity by the methods of Kirby-Bauer and two fold dilution. The crude acidic extracts only showed antibacterial activity against all six tested pathogens and the antibacterial ability of descending order is Aeromonas veronii, Staph epidermidis, Staph soparophytics, Aeromonas hydrophila, Citrobacter freundii, Staph warneri. The protein profiles of the acidic extracts mucus on tricine SDSgel showed proteins was less than 95 kD, and the highest levels was between 34 kD to 55 kD. Preliminar