PFOS对东亚三角涡虫HSP70蛋白及其基因表达的影响.pdfVIP

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN 年第 期 2014 10 PFOS 对东亚三角涡虫 HSP70 蛋白及基因表达的影响 宫晓宁  袁佐清  白芸 （山东理工大学生命科学学院，淄博 255049） 摘 要 ： 全氟辛烷磺酸 （Perfluorooctane sulfonate，PFOS）是一种持续的有机污染物，是全氟化合物 （Perfluorinated chemicals，PFCs）在环境中的代表性物质。以低等三胚层动物涡虫作为试验材料，使用Western blot 及RT-PCR 技术探究PFOS 对 涡虫HSP70 蛋白及基因表达的影响。结果表明，PFOS 短时间处理能诱导涡虫hsp70 mRNA 表达量升高，涡虫再生10 d mRNA 表达 受到抑制，HSP70 蛋白表达量在涡虫再生4 d 和7 d 时随PFOS 浓度显著上升，其它组变化不明显，PFOS 对mRNA 和蛋白表达量 趋势影响不一致，可能少量PFOS 能抑制HSP70 蛋白翻译，药物在涡虫体内积累量增加，蛋白高表达抵御毒性伤害。 关键词 ： PFOS 三角涡虫 HSP70 RT-PCR Western blot Effects of PFOS on Expression of HSP70 in Planarian Dugesia japonica Gong Xiaoning Yuan Zuoqing Bai Yun （School of Life Science ，Shandong University of Technogy ，Zibo 255049 ） Abstract: Perfluorooctane sulfonate（PFOS）is a persistent organic pollutant and has been found to be the predominant perfluorinated chemicals（PFCs）in the environment. In this study, we used planarians Dugesia japonica, which belong to the phylum Platyhelminthes, class Turbellaria, as an animal assay to evaluate the toxicological effects of PFOS on protein and mRNA expression of HSP70. The results indicated that the expression of hsp70 mRNA of planarians increased exposed to PFOS in short time and decreased when planarians regenerated of 10- days. However, protein expression of HSP70 increased significantly after regenerating planarians exposed to PFOS for 4- and 7-days, and other groups were not influenced significantly. The trend of mRNA expression were inconsistent with protern. It may be that little PFOS would inhibit the translation of HSP70, and the number of HSP70 increased to protect from toxicity of PFOS as that wa