枯草芽孢杆菌SNUB16对棉花种子萌发和幼苗生长的影响 毕业论文.docVIP

枯草芽孢杆菌SNUB16对棉花种子萌发和幼苗生长的影响 摘 要 为了探讨草芽孢杆菌SNUB16对棉花种子萌发和幼苗生长的影响以及抗枯萎病的效果，用不同浓度的枯草芽孢杆菌SNUB16菌液、棉花枯萎病菌菌液及它们的混合液分别处理棉花种子和土壤，做盆栽观察，记录各盆棉花出苗数、患病株数、幼苗鲜重和干重.试验结果表明，无论采用哪种接菌方法，枯草芽孢杆菌SNUB16均能增加棉花种子的出苗率，而且可显著降低棉花的患病率、增加棉花幼苗的鲜重和干重.接种棉花枯萎病菌后，棉花种子的出苗率显著降低，幼苗的患病率升高，幼苗的鲜重和干重显著降低.接种枯草芽孢杆菌SNUB16和棉花枯萎病菌的混合菌液,对棉花各项生理指标的影响与对照组差异不显著. 关键词 枯草芽孢杆菌SNUB16，棉花枯萎病菌，棉花，枯萎病，出苗率 Effect of Bacillus subtilis SNUB16 on seed germination and seedling growth of Cotton Plants (College of Life Sciences, Shaanxi Normal University, Xi’an 710062,Shaanxi) Abstract In order to explore the effects of Bacillus subtilis SNUB16 on cotton seed germination ,seedling growth and resistance to disease,I deal the cotton seed and the soil with different concentrations of Bacillus subtilis SNUB16 ,cotton Fusarium oxysporum bacteria and their mixture respectively.Then I make the pot experiment and record the basin potted, sick for cotton seedling tight, fresh and dry weight. The experimental results show that, no matter what the methods adopted, Bacillus subtilis SNUB16 can increase the seed germination rate, significantly reduce the prevalence of cotton and increase the cotton seedling fresh and dry weight .After inoculated against cotton Fusarium oxysporum bacteria, the cotton seed germination rate has reduced significantly , the morbidity of seedling has increased and the fresh and dry weight reduced significantly. Inoculated the cotton against Vaccination bacillus subtilis SNUB16 and cotton mixed bacteria of Fusarium oxysporum cotton, compared with the control, the difference of physiological indexes was not significant. Key words Bacillus subtilis SNUB16, Fusarium oxysporium, cotton, Fusarium wilt, Seedling rate. 近年来，由于棉花市场的开放，棉花价格上涨，播种面积不断增加，种植棉花已成为棉区农民增加收入的增长点.棉籽的发芽出苗，包括幼苗的生长繁殖深受棉花枯萎病的影响，连年出现大面积缺苗、死苗、低产现象，给农业生产带来严重的影响[1~2]. 棉花枯萎病病原菌是棉花枯萎病菌，属土壤传播的维管束病害，防治上有很大的难度.当前国内外在抗病育种、农业措施和化学防治等方面做了大量工作，但由于这些防病措施的种种弊病和不足，收效不尽人意 [3~4]，迄今尚缺乏理想的高产丰产品种和有效药剂，且化学药剂对环境的污染日益突出，生物防治日益受到重视[5~8].因此，了解棉花枯萎病菌的作用及其拮抗菌的作用机制，有着重要的意