铜胁迫下麻疯树的生理响应研究 毕业论文.doc

铜胁迫下麻疯树的生理响应研究 physiological response of Jatropha curcas researched under copper stress 摘 要 本试验利用不同浓度的硫酸铜溶液浇施麻疯树幼苗，旨在研究铜胁迫对麻疯树幼苗的外部形态和生理代谢的影响。结果表明：铜胁迫对麻疯树幼苗的茎围和株高生长有一定的抑制作用，在胁迫培养幼苗40天到60天过程中，叶片增长缓慢。麻疯树幼苗的生理生化指标变化表现为，丙二醛的含量随着处理浓度的增加而增加；可溶性糖、叶绿素的含量均比对照组明显低，叶绿素和可溶性糖的合成受到了抑制。春、秋两季相比较，春季麻疯树幼苗的叶绿素、可溶性糖含量相对较高，丙二醛相对较低。处理组的叶绿素含量在春季最低值为对照组含量的69.%；而秋季只有对照组的40.3%；丙二醛在铜离子浓度150mg/L时，达到最高值，春季丙二醛含量最高值是对照组的1.7倍，秋季最高值是对照组的1.8倍。试验证明，铜胁迫对麻疯树幼苗生长和生理代谢有一定抑制作用。 ABSTRACT The experiment researched morphologies of Jatropha curcas seedling and the effect of physiological metabolism in varied content of Cu(SO4)2. The showed the height and stem of Jatropha curcas would be inhibited by copper, leaves grew slow during culture.The content of MDA would increase with content of copper increase. The concentration of soluble sugar and chlorophyll would greatly reduce, and indicated the synthesis of chlorophyll and soluble sugar might be inhibited by copper. The content of two kinds things will change with seasons varied, in Spring which was higher than that in Fall.The experiment groups minimum content of chlorophyll was less than control group.In 150mmol/l Cu(SO4)2, content of MDA was the highest. MDA content in the spring of the highest value is 1.7 times of the control group, that of in autumn is 1.8 times of control. The result showed copper would inhibit the growth of Jatropha curcas seedling and physiological metabolism. Key words: Jatropha curcas;copper stress; chlorophyll; MDA 目 录 1.前言………………………………………………………………………………...1 2.材料与方法…………..…..………………………………………………….……..3 2.1材料…………………..….…………..……………..………………..……………3 2.1.1植株种子……………….……………....…………………………………….....3 2.1.2实验仪器…………………………...…..…………………………………...…..3 2.1.3实验药品…………..………………………………..…………………………..3 2.1.4培养液及培养基质…………………………..………..…………………..……3 2.2方法……………………………………………..……..…………………….……4 2.2.1种子的萌发实验………………………………..……..………………..………4 2.2.2幼苗前期培养…………………………………..………..……………..………4 2.2.3铜离子胁迫培养……………………………………..…..……………..………4 2.2.4指标测定……………………………………………..…..………..……………4 3.结