(完整)HIV__P24蛋白的原核表达及其单克隆抗体的制备__8.24修改.docVIP

HIV-1 P24蛋白的原核表达及单克隆抗体的制备 关键字：HIV-1 P24，原核表达，单克隆抗体，ELISA Expression of HIV-1 P24 protein and Preparation of its monoclonal antibodies WANG GONG ZE , LIU CHAOQI ,YANGJIANLIN,QINGXIAOLIN,LVBAIRUI Institute of Molecular Biology; Three Gorges University; Yichang HUBEI 443002; China ABSTRACT: Objective　To construct Prokaryotic expression plasmid of HIV-1 P24, purify recombinant HIV-1 P24 protein and prepare its monoclonal antibodies. Methods HIV-1 P24 cDNA was cloned into prokaryotic expression vector pET28a (+) and was further transformed into E.coli BL21(DE3). The recombinant HIV-1 P24 protein w