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* Map of cleavage sites of adcnovirus 2 DNA for restriction endonucleases EcoRI and HindIII Philip Sharp and David Baltimore * 电镜下Adenovirus mRNA和编码它的基因DNA的杂交 * * Structure of A Scientific Article Abstract (4 or 5 sentences) Introduction Background Problem You are able to address the problem Materials and methods Results My idea Detailed approaches Detailed results Conclusion Discussion Brief summary of the study and significance Related work implication and impact to the field future direction HUMAN HERPESVIRUSES Kaposi’s Sarcoma Virion Proteins of KSHV MCP TRI-1 TRI-2 SCIP SCAP ORF 33 ORF 21(TK) ORF 75 ORF 63 ORF 64 ORF 45 K 8.1 ORF 68 ORF 28 gBC gBN gN gL gM gH ORF 6 ORF 7 ORF 11 ORF 27 ORF 52 ORF 33 ORF 21(TK) ORF 75 ORF 63 ORF 64 ORF 45 Generation of ORF45-null Recombinant KSHV KanR selection Kan/SacB BAC36 ?ORF45 Generation of BACD45 KanR SucS Generation of Revertant Virus SucroseR KanS selection BAC36-Res ORF45 ?ORF45 SucR KanS SucR KanS Generation of BAC-STOP45 ORF45 ?ORF45 BAC36-Res BAC36-wt BAC36-Rev BAC-STOP45 BAC?45 M A M F V R T S S T H M A M F V R T S S T H M A M F V R T STOP CODON M A M F V R T S SacB Generation of Revertant and stop45 Viruses BAC36-wt BAC36-Rev STOP45 BAC?45 BAC36-wt BAC36-Rev STOP45 ?ORF45 KpnI NotI An ORF45-null Mutant Is Defective in Virion Release We also attempted to knockdown the endogenous KIF3A expression in BCBL-1 cells. A lentivirus based approach was employed to generate cells harboring the KIF3A shRNA sequences integrated into the host cell genome. Stable knockdown of KIF3A expression manifested as a highly significant reduction in viral egress. We also included a set of shRNAs against KHC (kinesin-1) in the studies to look into the role of KHC in KSHV transport. KHC has been implicated in transportation of HSV-1. KHC shRNA had little effect on KSHV virion production. However,t knockdown of KHC displayed little effects