SCI 论文写作剖析.ppt

HIV - 1 copy numbers 5,000 500 50 5 0 - - No. 1 No. 2 No. 3 SP NEG Actin-b Alu-PCR HIV-1 latent-infection in resting CD4 T-cells isolated from HIV-1 infected-individuals receiving suppressive HAART Combined miRNA antisense inhibitors can induce the viral production from latently-infectd resting CD4 T-cells isolated from HIV-1 infected individuals on HAART 骨干数据 ++ The miRNA antisense inhibitor-induced HIV-1 particles are replication-competent. mir-125b (red,MFE=-21.5), mir-150 (blue,MFE=-29.7) mir-125b (red,MFE=-21.5), mir-150 (blue,MFE=-29.7) mir-125b (red,MFE=-21.5), mir-150 (blue,MFE=-32.0) mir-125b (red,MFE=-21.5), mir-150 (blue,MFE=-29.7) mir-125b (red,MFE=-21.5), mir-150 (blue,MFE=-32.0) mir-125b (red,MFE=-21.5), mir-150 (blue,MFE=-33.6) mir-125b (red,MFE=-21.5), mir-150 (blue,MFE=-35.7) mir-125b (red,MFE=-21.5), mir-150 (blue,MFE=-31.1) mir-223 (MFE=-22.3) mir-223 (MFE=-22.3) mir-223 (MFE=-22.3) mir-223 (MFE=-20.6) mir-223 (MFE=-22.3) mir-223 (MFE=-20.2) mir-223 (MFE=-20.1) mir-223 (MFE=-23.6) mir-382 (MFE=-20.8) mir-382 (MFE=-20.2) mir-382 (MFE=-19.0) mir-382 (MFE=-20.2) mir-382 (MFE=-19.0) mir-382 (MFE=-19.0) mir-382 (MFE=-20.8) mir-382 (MFE=-24.0) mir-28 (MFE=-21.3) mir-28 (MFE=-21.3) mir-28 (MFE=-21.3) mir-28 (MFE=-20.5) mir-28 (MFE=-21.3) mir-28 (MFE=-21.3) mir-28 (MFE=-21.3) mir-28 (MFE=-23.3) 1156 1155 1156 1182 1185 1163 1158 1164 A. Binding site for mir-125b and mir-150 B. Binding site for mir-223 C. Binding site for mir-382 D. Binding site for mir-28 The conserved binding sites of cellular miRNAs in the 3’-end of various HIV-1 strains. The mechanism how cellular miRNAs inhibit the translation of HIV-1 proteins and contribute to the latency of HIV-1 in resting CD4 T-cells. Adapted from Han and Siliciano. Nature Medicine 2007. 13: 1138-40 统计学分析 样本量 统计方法 说服你自己 问泉那得清如许 为有源头活水来 实验生物学家 （Experimental biologist）： 是手巧的工匠，更是思想家 Biochemical Purification and Identification of APOBEC3G-Associ