专业英语微生物1.ppt

* 模板来自于 * Purification and Characterization of a Low-temperature Hydroxylamine Oxidase from Heterotrophic Nitrifier Acinetobacter sp. Y16* Introduction Autotrophic nitrifying bacteria perform aerobic nitrification, whereas heterotrophic nitrifying bacteria can simultaneously perform aerobic nitrification and denitrification. Hydroxylamine oxidation catalyzed by hydroxylamine oxidase (HAO) is considered as a key reaction of nitrification in autotrophic and heterotrophic bacteria. HAO in autotrophic bacteriaoxidizes hydroxylamine to nitrite, whereas the enzyme in heterotrophic bacteria oxidizes hydroxylamine to nitrite or to nitrous oxide. Acinetobacter sp. Y16 is a newly isolated heterotrophic nitrifying bacterium from the Songhua River in winter, which can degrade low amounts of ammonia nitrogen (5 mg/L) at 4-15 °C. Materials and Methods Cultivation Organism Preparation of Crude Enzyme Enzyme Purification AKTA Purifier Instrument、anion-exchange column、gel-filtration column、 TCA Enzyme Assays the absorbance change at 400 nm Effect of pH, Temperature, and Electron Acceptor on HAO Activity SDSand Molecular Mass Determination Bradford Purity Assay of the Purified Protein high performance liquid chromatography Amino Acid Sequence Analysis MS Results Purification of HAO  Activity Analysis of HAO Effect of pH, Temperature, and Electron Acceptor onHAO Activity Using cytochrome c as the electron acceptor to replace potassium ferricyanide in reaction mixtures, no HAO activity was observed. SDSAnalysis of HAO Amino Acid Sequence Analysis of Purified HAO * 模板来自于 *