基于CdTe@SiO纳米粒子和金纳米粒子体系荧光能量转移的开光模式检测三聚氰胺剖析.ppt

Effcient Fluorescence Energy Transfer System between CdTe-Doped Silica Nanoparticles and Gold Nanoparticles for Turn-On Fluorescence Detection of Melamine Feng Gao,* Qingqing Ye, Peng Cui, and Lu Zhang ■ INTRODUCTION ■ INTRODUCTION ■ INTRODUCTION AuNPs：high extinction coefficients——act as efficient acceptors for most fluorophores CdTe QDs：tunable, narrow, and symmetric emission spectra and broad excitation spectra——employed as donors in FET systems SiNPs： can efficiently reduce the outer environmental interference with fluorophores —— use silica as the shell and fluorophores as the core ■ INTRODUCTION ■ MATERIALS AND METHODS Instrumentation. Materials. Synthesis of AuNPs. Preparation of CdTe-Doped Silica Nanoparticles (CdTe@SiO2). Procedures for the Determination of Melamine. Instrumentation. LS-55 fluorescence spectrophotometer (PerkinElmer Co.) UV-3010 spectrophotometer (Hitachi, Japan). Scanning electron microscopy (SEM) (Hitachi, Japan) Hitachi H-600 transmission electron microscopy (TEM) (Tokyo, Japan). pHS-3c pH meter (Shanghai, China). Materials. Synthesis of AuNPs. Preparation of CdTe QDs Preparation of CdTe-Doped Silica Nanoparticles (CdTe@SiO2). Procedures for the Determination of Melamine. Procedures for the Determination of Melamine. ■ RESULTS AND DISCUSSION Morphological and Spectral Characterizations ofAuNPs, CdTe QDs, and CdTe@SiO2. Fluorescence Quenching Effect of AuNPs on CdTe@SiO2 Fluorescence “Turn-On” Assay of Melamine. Optimization of Experimental Conditions for the Detection of Melamine. Detection of Melamine. Effect of Potential Interfering Substances. Analysis of Melamine in Real Samples. Morphological and Spectral Characterizations of AuNPs Morphological and Spectral Characterizations of CdTe QDs Morphological and Spectral Characterizations of CdTe@SiO2 Morphological and Spectral Characterizations of CdTe@SiO2 Fluorescence Quenching Effect of AuNPs on CdTe@SiO2 Fluorescence Quenchin