棉花黄萎病菌的分离及培养技术研究.docVIP

编 号： 审定成绩： 重庆邮电大学 毕业设计（论文） 设计（论文）题目： 学院： 学 生 姓 名 ： 专 业 ： 班 级 ： 学 号 ： 指 导 教 师 ： 答辩组 负责人 ： 填表时间：年月 重庆邮电大学教务处制 棉花黄萎病（Verticillium Wilt of Cotton）是一种土传性的维管束系统病害，严重影响棉花产量全球因黄萎病所造成的经济损失平均每年达10亿多美元 【关键词】 液体培养基  ABSTRACT Verticillium Wilt of Cotton is one of the main diseases which affecting the yield and quality of the cotton. The key factor of the disease, in addition to less resistant varieties and appropriate climate conditions, the accumulation of a large number of Verticillium dahliae in soil is the main reason which cotton Verticillium wilt is popular. According to foreign reports, sleeping structure —— micro-sclerotia which Verticillium dahliae formed can survive in soil all the year round and become the most important infection source of against infection cycle. In the world, the economic loss caused by Verticillium wilt account for one billion U.S. Dollars average every year, one of the most cost-effective ways which control Verticillium wilt is filtering and using resistant varieties, while screening and identification of the species and related research all need to isolate and culture Verticillium species. In this study, according to search the existing literature with the existing experimental conditions, we collected farming soil and rhizosphere soil from cotton of Sichuan Jianyang as bacteria isolated material, and then gradually dilute the sample, and coating the dilution on the PDA medium. Based on the growth characteristics of colony, such as shape and color, we can pick a single colony and culture it on the medium. In this way, we can isolate and select the purified Verticillium dahliae. And then Verticillium dahliae were cultured with different liquid medium. So that selecting one of the most favorable medium for the growth of Verticillium dahliae, in order to culture the isolated and purified Verticillium dahliae into yello