SPE-HPL法测定藿香正气水中厚朴酚与和厚朴酚的含量.doc

SPE-HPLC法测定藿香正气水中厚朴酚与和厚朴酚的含量 郭怀忠1, 2，张斌1，王配1，景浩然1，王敏1 (河北大学药学院，河北 保定 071002河北省药物质量研究重点实验室，河北 保定 071002摘要：建立了SPE-HPLC法测定藿香正气水中厚朴酚与和厚朴酚含量的分析方法。采用硅胶SPE小柱提取纯化。色谱柱：C18（250 mm×4.6 mm I.D.，5 μm），以甲醇-水-乙腈-冰醋酸（70∶25∶5∶0.25）为流动相，流速为1.0 mL·min-1，检测波长为294 nm，柱温为室温。SPEHPLC法测得厚朴酚浓度在.0-80 μg·mL-1 (r=0.9998)，和厚朴酚浓度在.0~40 μg·mL-1 (r=0.9998) 范围内与峰面积线性关系良好。厚朴酚的平均回收率为99.8%，RSD为0.8%和厚朴酚的平均回收率为99.6%，RSD为0.5%。该方法快速、简便、准确、重现性好，能有效地控制藿香正气水的质量。 关键词：SPE HPLC Determination of Magnolol and Honokiol in Huoxiangzhengqi Water by SPE-HPLC GUO Huaizhong1, 2, ZHANG Bin1, WANG Pei1, JING Haoran1, WANG Min1 (1. School of Pharmacy, Hebei University, Baoding 071002; China; 2. Hebei Key Laboratory of Drug quality research, Baoding 071002; China) Abstract: A SPE-HPLC method for determining the contents of Magnolol and Honokiol in Huoxiangzhengqi Water was established. Samples were purified by silica gel SPE column. The HPLC column used in the experiment was C18 （250 mm×4.6 mm5 μm）,obile phase was methanol-water-acetonitrile-acetic acid (70: 25: 5: 0.25). The flow rate of the mobile phase was 1.0 mL·min-1 and detection wavelength was 294 nm. The experiment was accomplished at room temperature. It had good linear relationship between the peak areas of Magnolol and Honokiol and their concentrations at 4.0-80.0 μg·mL-1 (r=0.9998), 2.0-40.0 μg·mL-1 (r=0.9998), respectively. The average recovery of Honokiol was 99.6% with RSD being 0.5%, and that of Magnolol was 99.8% with RSD being 0.8%. The method was rapid, simple, accurate, with good reproducibility, and it can be used for quality control of Huoxiangzhengqi Water. Key words: SPE HPLC Huoxiangzhengqi Water Magnolol Honokiol 藿香正气水，功能解表化湿理气和中用于外感风寒、内伤湿滞或夏伤暑湿所致的感冒[1]具有抗菌、抗病毒、抗过敏、影响胃肠活动、肌肉松弛和中枢抑制等作用[2]《中国药典》2005年版一部[1]中以厚朴酚与和厚朴酚的含量作为藿香正气水的质量控制指标，以氯仿为溶剂，采用液液萃取技术对样品进行预处理，然后以高效液相色谱法(HPLC)进行含量测定。但液-液萃取预处理技术费时、易乳化、需要样品量大,并且需要有毒或有污染的有机溶剂，而用固相萃取可以避免缺点。固相萃取具有高的回收率和富集倍数，有机溶剂量少，无相分离操作避免产生乳化，分析物分易于收集，能处理小体积试样，操作简单快速等优点[]。[6, 7]采用高效液相色谱测定了藿香正气水中厚朴酚与和厚朴酚的含量还有对中白芷、橙皮苷、