Raybiotech蛋白质与抗体芯片在生物医学研究和转化医学中的应用讲课.pptxVIP

Scott Luo M.D.and Ph.D. R D Director sluo@ or sluo815@ Cell:(China) ;RayBio? C Series Array: Mouse Cytokine Antibody Array III;Overview: Protein Array Technology 蛋白/抗体芯片技术 Major components of protein arrays Classification of protein arrays Benefits of protein and antibody arrays Applications of protein and antibody arrays Biomarker discovery Drug discovery New insight on molecular mechanism;什么是蛋白质芯片？;蛋白质芯片制作;蛋白质芯片制作;化学发光检 测;固定在芯片上的生物材料;典型的抗体芯片（夹心法） 工 作 原 理;基于生物素标记的抗体芯片;; screening protein-carbohydrate interactions.  Identify the glycans binding partners in biological samples Identify whether target proteins are carbohydrate binding proteins Probe binding of viruses and whole cells to glycans Profile the substrate specificity of enzymes (glycosyltransferases, glycosidases, etc.) Profile the inflammatory immune response;蛋白质芯片的优势;The system CV for IL-5 from a 60-slide (960 arrays) run is about 9%;自动清洗系统 ;芯片的结果提取;膜芯片提取结果;;Normalization with IC data;Comparison data between US and GZ;;信号通路预测;Applications of RayBio? protein and antibody arrays RayBiotech生物高科技公司蛋白和抗体芯片的应用;;Cancer Cell 25, 605–620, May 12, 2014 (IF: 23.893);研究背景:;A Positive Feedback Loop between Mesenchymal-like Cancer Cells and Macrophages Is Essential to Breast Cancer Metastasis;研究内容; 乳腺癌细胞被诱导发生EMT时可以激活巨噬细胞分化为肿瘤相关性样的巨噬细胞;;A Positive Feedback Loop between GM-CSF from Cancer Cells and CCL18 from Macrophages In Vitro;Annals of Neurology, Volume 57, Issue 1, pages 67–81, January 2005;Is Autism an Inflammatory（炎症） Disease?;Vargas, et al. Ann Neurol 2005;57: 67-81.;(A) Cytokine protein arrays in CSF samples from an autistic patient and a control. The spots for macrophage chemoattractant protein (MCP)–1, interferon-, TGF-2, interleukin-8, and IGFBP-1 showed a marked density increase as compared with the CSF control. (B–I) Profile of expression (fold increase) of cytokines that were found markedly increased in autistic patients as compared with controls. p