Paralichthysorbignyanus.pptVIP

Salinity effects on osmoregulation and growth of the euryhaline flounder Paralichthys orbignyanus;Introduction;;Paralichthys orbignyanus;Distribution: Coastal and estuarine waters of Western South Atlantic Ocean (Brazil , Uruguay and Argentina) High market price. Wide tolerance to environmental factors such as salinity(0-40‰), pH , and nitrogenous compounds and acid stress. ;Materials and methods;Isosmotic point(等滲點)： 血漿中滲透壓與外界滲透壓相等的點。 Isoionic point(等離子點)： 血漿中離子濃度與外界離子濃度相同時的點。 ;Osmo- and ionoregulatory studies;馴養5天;在不同鹽度中馴養15天後的魚，以50ppm benzocain (苯坐卡因)麻醉，抽出1ml 的血，離心後，將血漿保存 在液態氮中。;Growth studies;Specific growth rate (SGR) =[( ln wf - ln wi ) / t x 100] wf = final weight (g) wi = initial weight (g) t = time (days). ;Blood：Osmolality and ionic composition (Na+ and Cl- ). Gill：Na+,k+ ATPase;Results;Osmo- and ionoregulatory studies;16;Isosmotic point = 328.6 mOsm kg-1 相當於 10.9 ‰;Isoionic point =210.8 mEq l-1 相當於11.6 ‰;Isoionic point = 136.8 mEq l-1 相當於 8.4 ‰ ;Isoionic point = 5.41 mEq l-1 相當於 22.6 ‰;Isoionic point = 5.21 mEq l-1 相當於15.7 ‰ ;;Growth studies;24;25;Discussion;27;The isosmotic point of P. orbignyanus was estimated as 328.6 mOsm kg-1 H2O and corresponded to 10.9‰ salinity. ;29;It is described that environment stressors such as salinity can effect fish growth (Borski et al.,1994).;;Conclusions; P. orbignyanus can be characterized as a  marine/estuarine euryhaline teleost capable of  carrying hyper/hypo iono- and osmoregulation  over the fluctuating salinity regime faced by  this species in the environment.;~The end~