NatalieD.Fedorova.pptVIP

Natalie D. Fedorova;Introduction;Outline;Reactions catalyzed by biotin-dependent enzymes:;Outline;Biotin carboxylase;Reactions catalyzed by subunits of biotin-dependent enzymes carboxyl transfer from biotin to the acceptor molecule;Outline;AccC - Biotin carboxylase;Biotin-dependent enzyme from Giardia intestinalis ● annotated as pyruvate carboxylase ● N-terminal portion is similar to acetyl-CoA and propionyl-CoA carboxylases, C-terminal portion is similar to the pyruvate carboxylase group (PycB-type) ● BCCP – shared by all biotin-dependant enzymes ● multi-domain enzyme with a unique domain organization;Outline;Carboxylases: carboxyltransferase domains ● acetyl-CoA carboxylase (ACCT): 2 subdomains A and D ● pyruvate carboxylase (PycB) Acetyl-CoA carboxylases;Pyruvate carboxylase (bacteria, eukaryotes);Methylmalonyl-CoA:pyruvate transcarboxylase ● found in Propionibacterium freudenreichii;Acetyl-CoA carboxylase;Pyruvate carboxylase;biotin-dependent enzyme from Giardia intestinalis range of the reactions catalyzed by BDE domains/subunits + reactions catalyzed by subunits operon organization of the corresponding genes 3 classes of BDE: domain/subunit organization phylogenetic analysis genomic context of the Giardia BDE conclusions ;10;10;PycB tree;Evolution of the BCCP domain fusions;ACCT-PycB protein from Giardia ● N-terminal portion is similar to acetyl-CoA carboxylases ● C-terminal portion is similar to the pyruvate carboxylases ● The same domain composition as in Propionibacterium freudenreichii ;ACCT-PycB protein from Giardia;Conclusions;Acknowledgements;Delineating domain hierarchies in the Conserved Domain Database (CDD) :;10