细胞色素P4501A1在白藜芦醇抑制人肝癌细胞增殖和促进其凋亡中的作用.pdfVIP

PCR blot CYPlAlmRNA的影响。蛋白印记分析 Western analysis 法检测 表达情况。结果：CCK．8检测结果显示，在体外培养状态下，白藜芦醇能 显著抑制人肝癌细胞SMMC．7721的增殖，在本实验的浓度剂量范围内， 随着白藜芦醇浓度的不断增大，抑制增殖的效果明显增强，呈浓度依赖关 系。同一浓度随着作用时间的不断延长，抑制增殖的效果明显增强，呈时 士1．51％、 65．51士2．41％ ，经统计分析得出，不同浓度白藜芦醇对人肝癌细胞 P 0．05 ，所以一定浓度白藜芦醇作用一定时间范围内，其对人肝癌细胞 SMMC．7721的增殖抑制作用存在浓度及时间依赖性。从人肝癌细胞 万方数据 醇作用72h后随药物浓度的升高抑制率斜率开始下降，说明单位时间内抑 SMMC．7721的抑制作用最为明显。后续实验以此作为作用截止点。 胞SMMC．7721细胞核呈现明显凋亡形态，随机对照组及空白组人肝癌细 胞SMMC．7721细胞核无明显变化。双染色流式细胞仪检测细胞凋亡率结 果显示：空白对照组、随机对照组、1009mol／L白藜芦醇实验组的凋亡率 CYPlAl蛋白的表达也呈现下降趋势。结论：体外培养状态下，白藜芦醇 能抑制人肝癌细胞SMMC．7721的增殖、促进其凋亡，其机制可能与白藜 芦醇在转录水平下调CYPlAl训卧 A的表达、从而在翻译水平减少 CYPlAl的蛋白表达有关。 关键词：白藜芦醇；人肝癌细胞SMMC．7721；细胞色素P4501A1 万方数据 Effectofresveratrolon of CYPlAl cytochrome expression inhuman 1 SMMC-772ceHs hepatoma livercancerwasoneofthemostcommon Abstract：0bjectivesPrimary tumorof tract．Ithave lesionsand malignant digestive highlymalignant easily tometastasis．Resveratrolwasakindof extractionfrom polyphenol plants，such as and hasbroad functions． polygonumcuspidatumgrapes．It pharmacological Resveratrolcan bloodvesselsand an rolein protect playimportantreducing bonecalciumlevel．Inrecent researchsshowedthat was years，many resveratrol theeffective for andheart componenttreatinglipid disease．Theliterature hastheeffectofantiliver reports，Resveratrol cancer,but themechanismWasnotclear．Theconventionalofantitumorwas way drugs inductionoftumorcell andinhibitthe oftumorcells． apoptosis proliferation This researchedtheinfluenceofResveratrolonthe experiment and ofhuman carcinomaSMMC-7721 cellsinvitro．In apoptosis hepatocellular orderto theeffectiveconcentration was identify usedtodetectthe range，CCK一8 effectofResveratrolonSMMC-7721 atdifferent proliferationinhibitory concentrations．Hoechst33258andAnnexinV／PI Kitwere to Apoptosisapplied detectthe ofResveratrolonSMMC·7721．Real time-PCR apoptosis technology wasusedtodetectionthe of CYP1A1 mRNA cytochrome change in 1 SMMC·772cellsbeforeandafter hepatoma blowing was to the of CYP1A1 applied analyze expressi