海洋放线菌分析.pptVIP

* * * 模板来自于 / * 模板来自于 * 模板来自于 / * 模板来自于 * 模板来自于 * 模板来自于 * 模板来自于 * 模板来自于 * 模板来自于 * 模板来自于 * 模板来自于 * Introduction 01 Materials and Methods 02 Results and Discussion 03 CONTENTS Contents 海绵动物 海绵动物是对一类多孔滤食性生物体的统称t Microorganisms serve as food particles, which are retained from seawater in the choanocyte chambers（细胞环室）, translocated into the mesohyl（中胶层） interior and digested by phagocytosis（吞噬作用）. Many sponges contain furthermore symbiotic microbial consortia（共生微生物） within their mesohyl matrix that may amount up to nearly half of their biomass（生物量）. Introduction Infectious disease is the number one cause of death in tropical countries accounting for approximately half of all fatalities. In addition, infectious disease mortality rates are also increasing in developed countries. Actinomycetes are of considerable interest owing to their ability to produce new chemical entities with diverse pharmacological activities Marine actinomycetes in particular have yielded numerous novel secondary metabolites Our aim is to isolate and culture actinomycetes from marine sponges and to characterize their potential to produce bioactive compounds,specifically those which inhibit the growth of human pathogens and parasites. Introduction Materials and Methods The first group of sponges (Aplysina aerophoba, Dysidea avara, D. tupha, Hemimycale culumella,Ircinia fasciculata) was collected by SCUBA diving at depths of 3–20 m in the Mediterranean Sea (Rovinj, Croatia, (GPS: 27?47.655 N; 34?12.904 W) in August 2008 The second group (Amphimedon sp., Callyspongia sp., C. aff. implexa, Hyrtios erecta, Negombata magnifica, Spheciospongia vagabunda) was collected at a depth of 10 m in the Red Sea (Ras Mohamed, Sinai, Egypt; (GPS: 27?47.655 N; 34?12.904 W) in August 2006 1. Sponge Collection Materials and Methods 2. Actinomycete Isolation Sponges were transferred to plastic bags containing seawater and transported to the laboratory. Sponge specimens were rinsed in sterile seawater, cut in