氨基酸工业菌种设计要点.pptxVIP

氨基酸工业菌种设计 孙际宾 中国科学院系统微生物工程重点实验室 中国科学院天津工业生物技术研究所 天津 滨海新区 国家自主创新示范区 自由贸易示范区 55高教机构 150世界500强企业 6万家中小企业 全国最快的经济增长率 HDI全国第三 -UNDP 最宜居的城市 交通最便利的城市 top 3 most dynamic cities in the world-McKinsy Suzhou Cofounded by CAS and Tianjin Officially approved 2012.11.29 480 persons (800 in 5 years) 3 departments/key labs 4 core facilities 33 PIs New RD model Tianjin Institute of Industrial Biotechnology, CAS 解决“生物工具设计和生物体功能利用”关键问题，发展工业生物技术创新体系，促进工业生物技术的创新与成果转化 Systems and Synthetic Biotechnology Group Genomics Time-series omics Biological database Metabolic network reconstruction Pathway design Enzyme design Systems metabolic engineering Reverse metabolic engineering High throughput Screening Amino acids Organic acids Why acids? Biocompatability (physical and chemical) Tolerance ? monohydric alcohol Chemical stability ? aldehyde, C=C Physical stability ?hydrocarbon Solubility Biological relevance ?efficiently evolved Yield Oxygen-enriched, life likes oxidation Application Food, feed, healthcare, pharmaceutical, material, intermediate Highly scalable in chemical industry Inherently suitable for bioproduction Easy to build competence against chemistry More than 50% of world production capacity 60 billion RMB, 25% of Chinese fermentation industry (excluding fuel ethanol, alcohol drinks, beverages) Representative of Chinese Fermentation Industry 6 Challenge: Production capacity excess 113 破产？停产？转产？ 纠纷？ 7 Lower yield, titer and productivity Averagely 20% lower Challenge: low competitiveness Yield 1% up ? cost 70-100 rmb down Titer 1% up ? cost 40-60 rmb down Fermentation 1h ? cost 15-20 rmb down 100K tons 1% yield up Saving feedstock 2500 tons, cost 10m RMB down Lysine factory 8 Challenge: the IP issue Ajinomoto Evonik Degussa China 450 lysine patent family After many years of traditional planned economy, the Chinese amino acid industry has serious IP problem. National needs Promote existing industries IP, yield, cost, competativeness Develop new potentials Derivatives New amino