nationofmitochondriallocalizationofBmHSP20.8protein.ppt

The determination of mitochondrial localization of BmHSP20.8 protein in BmN cells. Chengcheng Wu et al. J Insect Sci 2015;15:99 ? The Author 2015. Published by Oxford University Press on behalf of the Entomological Society of America. The determination of mitochondrial localization of BmHSP20.8 protein in BmN cells. EGFP, BmHSP20.8 and their fusion protein, BmHSP20.8-EGFP and EGFP-BmHSP20.8, were identified in the cytoplasmic or mitochondrial portion of BmN cells by western blotting using the HIS monoclonal antibody. The recombinant BmHSP20.8 and EGFP-BmHSP20.8 but not BmHSP20.8-EGFP could be identified in mitochondrial extracts, suggesting mitochondrial localization and a localization signal domain in the C-terminal of BmHSP20.8.