分子印迹流动注射化学发光法测定盐酸强力霉素(精选).docVIP

分子印迹流动注射化学发光法测定盐酸强力霉素 Determination of doxycyclinehydrochloride molecular imprinting chemiluminescence method 周艳梅1，张成丽1，雷建都2，马同森1，马光辉2，苏志国2，晁阳1 Zhou Yanmei 1, Zhang Chengli 1, Lei Jian 2, Ma Tongsen 1, Ma 2 glorious, Su Zhiguo 2, Chao Yang 1 1．河南大学化学化工学院。河南开封475004 College of chemistry and chemical engineering, Henan University 1. Henan Kaifeng 475004 2．中国科学院过程工程研究所，生化工程国家重点实验室，北京100080 2 Chinese Science Institute of process engineering, State Key Laboratory of biochemical engineering, Beijing 100080 摘要研究发现在碱性条件下，盐酸强力霉素对鲁米诺一铁氰化钾体系化学发光反应具有明显的增敏作 The study found that in alkaline conditions, doxycycline hydrochloride has obvious enhancement of luminescence reaction Lumino ferricyanide system of Chemistry 用，据此建立了分子印迹一流动注射化学发光法定鼍分析盐酸强力霉素的新方法。以甲基丙烯酸为功能单体、 Using the molecular imprinting, a new flow injection chemiluminescence method for the analysis of legal alligator doxycycline hydrochloride. Methacrylic acid as the functional monomer, 乙二醇二甲基丙烯酸酯为交联剂合成了盐酸强力霉素的分子印迹聚合物。以此分子印迹聚合物为分子识别 Two glycol methacrylate synthesis of molecularly imprinted polymer of doxycycline hydrochloride as crosslinking agent. The molecularly imprinted polymer for molecular recognition 物质，利用盐酸强力霉素一鲁米诺一铁氰化钾化学发光体系，结合流动注射化学发光分析技术，建立了测定盐 The material, use a doxycycline hydrochloride a luminol Potassium Ferricyanide Chemiluminescence System, chemiluminescence analysis with flow injection technique was developed for the determination of chemical, salt 酸强力霉素高选择性的分子印迹一流动注射化学发光分析方法。方法的线性范围为9．0×10叫～6．0×10_5 g Acid doxycycline highly selective molecularly imprinted a flow injection chemiluminescence analysis. The linear range of the method is 9 × 10 ~ 6 × 10_5 g called ·rnL～，检出限为3．2×10q g·rrlL～。对6．0X10叫g·rnL-1的盐酸强力霉素水溶液进行分析，9次平行 · rnL ~, the detection limit is 3.2 × 10q g · rrlL ~. Water doxycycline hydrochloride solution on 6.0X10 called G and rnL-1 were analyzed, 9 parallel 测定的相对标准偏差为3．5％。利用此方法测定尿样和盐酸强力霉素药片中盐酸强力霉素的含量，结果令人 The relative standard deviation is 3.5%. Content determination of doxycycline hydrochlo