chapter 6 MHC.ppt

Section 4 Bind of antigenic peptides and MHC molecules 抗原肽 MHC T 细胞 抗原递呈细胞（APC） 1 Peptide-binding cleft Peptide-binding cleft Bound peptide (2) Region II of HLA genes Class II RING DP DM LMP2 LMP7 DQ DR B2 A2 B1 A1 A B TAP1 TAP2 B2 A2 B3 B1 A1 B* A Three loci, DP, DQ and DR; Code for? one alpha- and one beta-chain polypeptide, which associate together to form the class?II antigens b1 b2 a1 a2 HLA-DP,DQ,DR (3) non-HLA genes Others Region III of HLA genes Not expressed on the cell surface Unrelated with the transplantation Related with immune response DMA and DMB gene loci code a a chain and b chain respectively，which play an important role in the assembling of MHC-II. LMP-2 and LMP-7 code a polypeptide, which is portion of the proteasome. TAP-1 and TAP-2 codetransporter in antigen processing，TAP. RING DP DM LMP2 LMP7 DQ DR B2 A2 B1 A1 A B TAP1 TAP2 B2 A2 B3 B1 A1 B* A Processing and presentation of the endogenous antigen Surface of the cell Golgi complex Rough endoplasmic reticulum Cytoplasm Endogenous antigen proteasome Calreticulin Antigenic peptide Surface of the cell Rough endoplasmic reticulum Processing and presentation of the exogenous antigen Exogenous antigen Early endosome Late endosome Antigen degradation fusion Golgi complex MHC-I molecule chain The difference between two pathways of antigenic processing and presentation MHC-II molecule MHC- I molecule source of the antigen Enzyme for protein process Cell for antigen process Binding side for MHC antigenic peptide MHC molecule Presentation object Exogenous antigen lysosomal enzyme Professional APC phagolysosome II CD4+T cell Endogenous antigen proteasomes All nuc