广西莪术快速繁殖和后代植株性状调查的研究.pdf

单株郁金个数分别为40个、35个和31个，其单株郁金重量和单株郁金个 数均高于以根茎繁殖的对照植株。 3、品质分析：组培处理中，莪术多糖的提取率以METl．钿g／L处理的 再生植株最高，达2．15％。块根中，无机元素含量比较，CCIOOmg／L壮苗处 理的再生植株Cu含量最高，为7．75p e／g；METl．Omg／L壮苗处理的再生 植株的Fe和‰含量最高，分别为94．50p e．／g和109．42lle／g；对照植株 的Zn、Ca和一g含量最高，分别为72．42lI g／g、602．33|I g／g和392．00 P g／g。总体而言，METl．Onlg／L壮苗处理的再生植株的6种元素含量相对 较高，与对照接近，可较好的保证产品质量。 ‘ 综上所述，以№为基本培养基，附加BA3．Ong／L为不定芽增殖和继代培 养配方；附力QBA3．Omg／L及METl．Owng／L为试管苗壮苗培养配方；附加 NAA2．Omg／L为试管苗生根配方．此外，经METl．OeIg／L壮苗处理后的再生植 株后代性状较好，对产量和品质的提高较有利。通过试验，可建立广西莪 术快速繁殖体系，实现组培苗商品化、工厂化生产。 关键词：广西莪术快速繁殖后代植株性状 STUD I ES 0N RAP l D PROPAGAT l 0N AND CHARACTERS OF THE SUBSEQUENT GENERAT I ONS OF CURCUMA KWANGS I ENS I S ABSTRACT Since the consecutive dredging for many ycars,wild resources began to reduce the number of Curcuma kwangsiensls S．G Lee et C．F．Liang．And germplasm began to decline after a long-term asexual reproduction,the yield reduced and the quality debased．The major object of this study is to establish the rapid propagation system of Curcuma kwangsiensis,and provide theory for industrial propagation in future． The experiment with rhizomtous of Curcuma kwangsiensis S．G．Lee et C．F．Liang as explant,carried OU adventitious buds induction,multiplication,seedling cultivation, rooting and transplantation,established the Rapid Propagation system of Curcuma kwangsiensis．S．G．Lee et C．F．I．tang． The major results were嬲following： 1． Rapid Propagation：The optimizing medium for multiplication was MS+BA3mg／L+sucrose 3％+agarO．4％：and MS+BA3mg／L+MLr1．0mg／L+sucrose 3％+agar0A％WaS goodfor seedlingcultivation；theoptimizingmediumofrootingwas MS+NAA2．0mg／L+sucrose 3％+agar0．4％．Transplant survival rate was the highest when the test·tIIbe plantlets were treated with METl．0 mg／L in seedling cultivation, reached 100％． 2．Agronomy Characters Research：Among the subsequent generations, supplemented with METl．0mg／L induced the highest height囊s 89．7cm,and Chlorophyll eontent WaS maximal also,as 1．235 mg／g．The enzyme strip of isozyme of peroxide showed that there WaS nO variation with