Natural incidence of endophytic bacteria in pea cultivars under field conditions.doc

Natural incidence of endophytic bacteria in pea cultivars under field conditions 作者: Elvira-Recuenco M, van Vuurde JWL 来源出版物: CANADIAN JOURNAL OF MICROBIOLOGY????卷: 46????期: 11????页: 1036-1041????出版年: NOV 2000 被引频次: 45 参考文献: 29 引证关系图 摘要: Pea plants grown in the field were used to study the natural incidence of endophytic bacteria in the stem. Eleven pea cultivars at the flowering stage were screened for the presence of endophytic bacteria using a printing technique with surface disinfested stem cross-sections on 5% Trypticase Soy Agar TSA . Five cultivars showed colonization. Cultivar Twiggy showed the highest and most consistent colonization and was further investigated. Stems of cv. Twiggy at the pod stage were analyzed for endophytic bacterial types and populations. Cross-sections of surface disinfested stems were printed on 5% TSA. Endophytic bacterial populations decreased from the lower to the upper part of the stem. One section from the third and the fourth internode was surface disinfested, homogenized, and spiral plated on the media 5% TSA, R2A, and SC Davis et al. 1980 . Over a series of 30 samples, 5% TSA gave significantly better recovery of bacterial endophytes compared with R2A and SC media. For most stems, populations ranged from 10 4 to 10 5 CFU/g except in one of the field blocks in which endophyte populations were uniformly higher. Comparison of colony counts by spiral plating and printing showed a positive correlation. The most frequently recovered bacterial types were Pantoea agglomerans and Pseudomonas fluorescens. Less frequently isolated were Pseudomonas viridiflava and Bacillus megaterium. 文献类型: Article 语言: English 作者关键词: endophytic bacteria; pea; stem colonization; cultivar screening; biodiversity KeyWords Plus: BIOLOGICAL-CONTROL; FUSARIUM-WILT; PLANT-GROWTH; ROOT-ROT; PSEUDOMONAS; COTTON; RHIZOSPHERE; RESISTANCE; INDUCTION; DISEASE 通讯作者地址: van Vuurde, JWL 通讯作者 , Plant Res Int, POB 16, NL-6700 AA Wageningen, Netherlands