Chp11 Analysis of Antibiotics.ppt

Chapter 11 Analysis of Antibiotics SECTION 1 GENERAL CONSIDERATIONS There are more than 100 types of Antibiotic drugs and their preparations 1) there are some impurities in general products of antibiotics, fine purified 2)?most of antibiotics are unstable 3) decomposition products of antibiotics are less or no therapeutic efficacy and more side-effect or toxic Besides the limit tests and methods for quantitative determination the procedures of assay are also specified. 1. General examinations for antibiotics: ①? Identification ②??Undue toxicity ③??Sterility ④??Pyrogens ⑤??Water content ⑥??Clarity of solution ⑦??pH value of solution ⑧ Depressor substance ⑨ Assay 2. Assay: Biological or physical-chemical procedure 1) Biological procedure ① the principle comply with the clinical requirement ②?the?sensitivity is high ③ the amount of sample needed for assay is small ④ to estimate the total antibiotic activities 2)?Physical-chemical procedure: ①?the process is more easy than the biological procedures ② the result is more precise and accurate The results may only represent total amount of antibiotics, not bio-potency value Only the results of physical-chemical method are complied with of biological method can it be used in Assay 3. Content of this chapter: 1) ?-lactams 2) Aminoglycosids 3) Tetracyclines Section 2 ?-LACTAM ANTIBIOTICS 1. chemical structure and properties 1) structure ① penicillin Section 2 ?-LACTAM ANTIBIOTICS 1. chemical structure and properties 1) structure ① penicillin Section 2 ?-LACTAM ANTIBIOTICS 1. chemical structure and properties 1) structure ① penicillin RCO-： ② cephalosporin 2) properties ①??acidic action