山羊IGFBP-6因的克隆及其组织表达的发育性变化-游杰.docVIP

山羊IGFBP-6基因的克隆及其组织表达的发育性变化 动物科学（特种经济动物养殖方向） 2008级 指导摘要：IGFBPs在体内循环系统中能与IGFs结合，延长IGFs的半衰期，调节IGFs的代射清除率，提高IGFs的生物学效应。肝脏和局部组织分泌的IGFBPs共同调控各个组织的生长与发育，从而促进动物机体的生长。本研究以培育品种南江黄羊为试验材料，应用RT-PCR和克隆技术克隆IGFBP-6基因的全编码区序列采荧光定量PCR技术研究在南江黄羊生后5个时间点0d、30d、60d、90d、120d组织肝脏、肺、心脏、背最长肌、半膜肌和臂三头肌的发育性变化。结果表明：克隆得到南江黄羊IGFBP-6基因的CDS全序列长度为711bp，编码235个氨基酸基因的核苷酸、氨基酸序列绵羊的一致性为97%，与其他物种间的一致性也较高在各个时间点的各个组织中都检测到IGFBP-6基因的表达，但相对表达量存在一定的差异。IGFBP-6在背最长肌的基因表达量远远高于肝脏、心脏、脾脏、肺脏和其它肌肉等组织的表达量（0.01P0.05或P0.01），在肝脏中的表达量最低。 关键词：南江黄羊；IGFBP-6基因；克隆；荧光定量PCR；表达 Cloning of IGFBP-6 Gene and its Developmental Change in Tissue Expression You Jie, Animal?science (Special?economical?animal breeding), Grade 2008 Supervised by Professor ZHANG Hongping Abstract:IGFBPs (insulin-like growth factors, IGFs) family play an important role in maintaining normal physical activities and processes, including embryo differentiation, body development, sugar, lipid and protein metabolism. IGFBPs can prolong the half-life of IGFs, enhance its biological activity and regulate its metabolism clearance by binding IGFs in circulation system. IGFBPs secreted from liver and other local tissues can regulate and stimulate growth of tissues corporately. In this study, Nanjiang yellow goat was choose as experimental material. RT-PCR and cloning technology were employed to clone the full coding sequence of IGFBP-6 genes out the total RNAs extracted from liver tissue at 0d after birth, followed by using of Q-PCR to identify the profile of gene expression in different tissues(liver, lung, heart, longissimusdorsi, semimembranosus, musculustriceps) and at different time points(0d、30d、60d、90d、120d after birth). The results indicate:(1) We have cloned the CDS full sequence of IGFBP-6 (711bp), which encoding a peptide consists of 235 amino acid residues. The nucleotide and amino acid sequence of IGFBP-6 possess high homogeneity with sheep-about 97% of its sequence -as well as other spices; (2)We have detected the existence of IGFBP-6 mRNA in all tissues a