CancerImmunotherapy-LongwoodUniversity.pptVIP

Cancer Immunotherapy Manipulation of co-stimulatory signals Enhancement of APC activity Cytokine therapy Monoclonal antibody therapy Co-stimulatory signals Possibility of transfecting irradiated inactivated tumor cells with B7 co-stim genes Perhaps a similar vaccine may prevent metastasis after surgical removal of tumors Enhancement of APC activity Transfect tumor cells with gene encoding GM-CSF ? reinfuse to patient to stimulate DC’s to enhance Ag presentation to TH’s and Tc’s Or culture patient DC’s w/ GM-CSF, TNF-a, and IL-4 ? expand DC population Or use adjuvants of Mb, Cp to stimulate M? to better activate TH cells Cytokine therapies From cloned genes for various cytokines IFN’s, TNF’s, etc. ….still a work in progress IFN’s – daily injections of IFN-a – partial/complete tumor regression All 3 types of IFN MHC I on tumor cells; CTL response All 3 appear to inhibit cell division in tumor cells IFN-y stim activity of Tc’s, M?, and NK cells Cytokine therapies cont’d  TNF’s – both TNF-a/B show direct effects on tumor cells, while sparing normal cells TNF-a shows anti-angiogenic affects to tumors ↓blood flow and oxygen Monoclonal antibodies * Also, melanoma antigens shared by a number of different tumors *