辽河保护区五种委陵菜在PEG胁迫下渗透调节物质可溶性蛋白质与脯氨酸变化.docVIP

辽河保护区五种委陵菜在PEG胁迫下渗透调节物质可溶性蛋白质与脯氨酸变化 　　摘要：采用聚乙二醇模拟干旱胁迫，研究5种委陵菜（Potentilla）抗旱性，探索干旱胁迫对5种委陵菜属植物渗透调节物质可溶性蛋白质与脯氨酸含量的影响。结果表明，干旱胁迫处理后委陵菜可溶性蛋白相对含量有所增加，干旱胁迫1～5 d莓叶委陵菜可溶性蛋白相对含量增幅最大，处理7 d蛇含委陵菜可溶性蛋白质相对含量增幅最大，而蔓委陵菜与三叶委陵菜可溶性蛋白质相对含量增幅较小，且在20%PEG处理第5天后下降。干旱胁迫处理使5种委陵菜叶片脯氨酸相对含量上升，其中蛇含委陵菜与莓叶委陵菜脯氨酸相对含量增幅最大；10%PEG与15%PEG胁迫处理后5种委陵菜脯氨酸相对含量均呈上升趋势，20%PEG胁迫处理第5天伏委陵菜与三叶委陵菜叶片中的脯氨酸相对含量即呈现下降趋势。5种委陵菜抗旱能力依次为蛇含委陵菜莓叶委陵菜蔓委陵菜伏委陵菜三叶委陵菜。 　　关键词：委陵菜（Potentilla）；干旱胁迫；可溶性蛋白质；脯氨酸 　　中图分类号：Q945 文献标识码：A 文章编号：0439-8114（2016）08-1936-03 　　DOI：10.14088/j.cnki.issn0439-8114.2016.08.009 　　Abstract： In this paper， utilizing polyethylene glycol to simulate drought stress，drought resistance was studied on 5 species of Potentilla. The influence of drought stress on the content of soluble protein and proline was also studied.Under the drought stress，soluble protein content of 5 species of Potentilla increased. From 1 day to 5 days， the soluble protein content of Potentilla fragarioides shows the largest increase.In 7 days， the protein content of Potentilla kleiniana increased most. in the soluble protein content of Potentilla flagellaris and Potentilla freyniana increased less， which decreased in 5 d after treated by 20% PEP. Under drought stress，proline content of 5 Potentilla increased.In Potentilla kleiniana and Potentilla fragarioides，the proline content increased most.Also，the proline content of 5 species of Potentilla increased after treated by 10% and 15% PEG. Treated by 20% PEG，the relative content of proline in Potentilla paradoxa and Potentilla freyniana decreased after 5 days. The drought resistance of the five Potentilla is gauged as follows： Potentilla kleiniana， Potentilla fragarioides， Potentilla flagellaris， Potentilla paradoxa， Potentilla freyniana. 　　Key words： Potentilla；drought stress；soluble protein；proline 　　辽河是中国七大江河之一，发源于河北省七老图山脉之光头山，流经河北、内蒙古、吉林、辽宁，至盘锦注入渤海，流域面积 21.96×104 km2，全长 1 345 km。由于长期开垦耕作，导致河岸带沙化、盐碱化，水土流失、风沙侵蚀日益严重，生态环境脆弱，已经成为制约其生态环境恢复、生态产业发展的重要因素之一[1]。辽河保护区是2010年3月经中国共产党辽宁省委员会、辽宁省