pIRES2-DsRed-Express载体.docVIP

pIRES2-DsRed-Express 载体 出品公司: Clontech 质粒类型: 哺乳动物表达载体 启动子: CMV 表达水平: 高 克隆方法: 多克隆位点，限制性内切酶 载体大小: 5264 bp 查看载体序列 5' 测序引物及序列: CMV-F: CGCAAATGGGCGGTAGGCGTG 3' 测序引物及序列: IRES-R: CCTCACATTGCCAAAAGACG 载体标签: C-DHFR mouse DHFR 载体抗性: 卡那霉素 筛选标记: 新霉素 pIRES2-DsRed-Express 质粒图谱 载体描述 pIRES2-DsRed-Express contains the internal ribosome entry site IRES; 1, 2 of the encephalomyocarditis virus ECMV between the MCS and a mutant of the Discosoma sp. red fluorescent protein DsRed-Express coding region 3–5 . This permits both the gene of interest cloned into the MCS and the DsRed-Express gene to be translated from a single bicistronic mRNA. pIRES2-DsRed-Express is designed for the efficient selection by flow cytometry of transiently transfected mammalian cells expressing DsRed-Express and the protein of interest. This vector can also be used to express DsRed-Express alone or to obtain stably transfected cell lines without timeconsuming drug and clonal selection. DsRed-Express is a human codon-optimized 4 DsRed variant of Discosoma sp. red fluorescent protein 6 . Its coding sequence contains nine amino acid substitutions which improve solubility of the protein and reduce the time from transfection to detection of red fluorescence exitation and emission maxima 557 nm and 579 nm, respectively . In addition, these substitutions reduce the level of residual green emission 5 . Although DsRed-Express most likely forms the same tetrameric structure as wild-type DsRed, DsRed-Express displays a reduced tendency to aggregate 3, 5 . The MCS in pIRES2-DsRed-Express is between the immediate early promoter of cytomegalovirus P CMV IE and the IRES sequence. SV40 polyadenylation signals downstream of the DsRed-Express gene direct proper processing of the 3' end of the bicistronic mRNA. The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T antigen. A neomycin-resistance cassette Neo r , consisting of the SV40 early promo