各类质谱的优缺点.docVIP

各类质谱的优缺点 1.磁质谱 Benefits ??Classical mass spectra Very high reproducibility Best quantitative performance of all mass spectrometer analyzers ?High resolution High sensitivity ?High dynamic range ?Linked scan MS/MS does not require another analyzer ?High-energy CID MS/MS spectra are very reproducible Limitations ?Not well-suited for pulsed ionization methods e.g. MALDI Usually larger and higher cost than other mass analyzers ?Linked scan MS/MS gives either limited precursor selectivity with unit product-ion resolution, or unit precursor selection with poor product-ion resolution Applications ??All organic MS analysis methods ?Accurate mass measurements ?Quantitation Isotope ratio measurements 2.四极杆质谱 Benefits ?Classical mass spectra ?Good reproducibility Relatively small and low-cost systems ??Low-energy collision-induced dissociation CID MS/MS spectra in triple quadrupole and hybrid mass spectrometers have efficient conversion of precursor to product Limitations ?Limited resolution Peak heights variable as a function of mass mass discrimination . Peak height vs. mass response must be tuned. ?Not well suited for pulsed ionization methods Low-energy collision-induced dissociation CID MS/MS spectra in triple quadrupole and hybrid mass spectrometers depend strongly on energy, collision gas, pressure, and other factors. Applications Majority of benchtop GC/MS and LC/MS systems Triple quadrupole MS/MS systems ?Sector / quadrupole hybrid MS/MS systems ?Quadrupole/ion trap hybrid MS/MS systems ?Quadrupole/FTICR hybrid MS/MS systems 3.飞行时间质谱 Benefits ?Fastest MS analyzer Well suited for pulsed ionization methods method of choice for majority of MALDI mass spectrometer systems High ion transmission MS/MS information from post-source decay ?Highest practical mass range of all MS analyzers Limitations ?Requires pulsed ionization method or ion beam switching duty cycle is a factor Fast digitizers used in TOF can have limited dynamic range Limit