Oxygenconsumptionbydigitonin-permeabilizedskin.pptVIP

Oxygen consumption by digitonin-permeabilized skin fibroblasts Measurements were performed using 0.45–0.62?mg of cell protein/ml and 0.1?mg of digitonin/mg of protein. Pavel JE?INA et al. Biochem. J. 2004;383:561-571 ?2004 by Portland Press Ltd Oxygen consumption by digitonin-permeabilized skin fibroblasts Measurements were performed using 0.45–0.62?mg of cell protein/ml and 0.1?mg of digitonin/mg of protein. Subsequent additions of pyruvate 5?mM , malate 1.5?mM , succinate 10?mM , ADP 0.5?mM , oligomycin 1?μM , FCCP 1?μM , antimycin A 0.2?μg/ml , ascorbate 5?mM , TMPD 1?mM and KCN