I型胶原-壳聚糖合材料生物相容性研究.docVIP

I型胶原-壳聚糖复合材料的生物相容性研究 张永强1 任志鹏1 杨自权1 孙晓丹2 卫小春1 1山西医科大学第二医院骨科骨与软组织损伤修复山西省重点实验室 030001 2清华大学材料工程学院 【摘要】　目的　评价I型胶原-壳聚糖复合材料作为组织工程支架材料的生物相容性。方法　体外培养兔骨髓间充质干细胞（BMSCs），采用四甲基偶氮唑盐（MTT）比色法检测不同分组的BMSCs生长情况，酶标仪测吸光度值（OD），计算细胞相对增值率（RGR％）；利用材料浸提液与兔新鲜血混合观察红细胞溶解情况，评价材料细胞生物相容性。结果　空白组（A组），50％浓度组（B组）和100％浓度组（C组）BMSCs均生长良好，并于1、3、5天逐渐增殖，阳性对照组（D组）细胞于第１天开始变圆、皱缩，3天和5天均皱缩死亡。A、B、C三组细胞在第1、3、5天各组吸光度值无显著差异（P 0.05），但均与Ｄ组有明显统计学差异（P 0.05）。材料相对溶血率为4.1％。结论　I型胶原-壳聚糖复合材料无明显细胞毒性，有良好的生物相容性。 【关键词】　胶原-壳聚糖材料；细胞毒性试验；溶血试验 Biocompatibility researches of type I collagen - chitosan scaffold Zhang yongqiang1, Ren zhipeng1, Yang ziquan1 et al.1 Department of Orthopedics,the second hospital of Shanxi Medical University, bone and soft tissue healing Key Laboratory of Shanxi Province; 2 Tsinghua University, Institute of Materials Engineering [Abstract] Objective To evaluate the biocompatibility of the type I collagen - chitosan scaffold for tissue engineering. Methods Cultured bone marrow mesenchymal stem cells BMSCs in 4 different groups A,B,C and D .Used tetrazolium salt MTT colorimetric to assay the growth of different groups,and measure the optical density OD by microplate reader, then calculate the relative growth rate RGR% .Mixed the scafford extracts with fresh blood of the rabbit,the grade of hemolysis was made in the measures of optical density at 492nm. Results BMSCs of group A, group B and group C were growing well, and proliferating gradually in the 1st,3rd and 5th day. The positive control group D cells rounded and wrinkled in the 1st day, then, cells were shrinkage death. The optical density were no significant statistical differences between group A,B and C P 0.05 , but all had significant statistical differences with group D P 0.05 .Hemolysis percentage of the scafford was 4.1%. Conclusion I collagen - chitosan scafford has no significant cytotoxicity, and accords with the biomaterial standards of tissue engineering. [Key words] I collagen-chitosan scafford; Test of hemolysis；Test