andVDAC2fromporcineoocytesDetectionofVDACisoform.pptVIP

Immunoblotting of VDAC1 and VDAC2 from porcine oocytes Detection of VDAC isoforms in porcine GV immature and MII stage mature oocytes. María?Carolina Cassará et al. Biosci. Rep. 2010;30:193-200 ?2010 by Portland Press Ltd Immunoblotting of VDAC1 and VDAC2 from porcine oocytes Detection of VDAC isoforms in porcine GV immature and MII stage mature oocytes. Proteins of 300 oocytes per lane were separated by SDS/PAGE 12% gel and transferred to nitrocellulose membranes. Membranes lanes 1 and 2 were incubated with anti-VDAC1 antibody AS P1/6, 1:100 . A second set of membranes lanes 3