gelscanbeusedtotestinteractionsbetweenHDCisoforms.pptVIP

Semi-denaturing gels can be used to test interactions between HDC isoforms and L-histidine substrate analogues A Samples of 200?μg of recombinant HDC1/516–His were incubated in the absence Control or presence of 1?mM α-FMH, 20?mM L-histidine His or 20... John?V. FLEMING et al. Biochem. J. 2004;381:769-778 ?2004 by Portland Press Ltd Semi-denaturing gels can be used to test interactions between HDC isoforms and L-histidine substrate analogues A Samples of 200?μg of recombinant HDC1/516–His were incubated in the absence Control or presence of 1?mM α-FMH, 20?mM L-histidine His or 20?m