MM-CKcontentandcatalyticactivityweredecreasedduring.pptVIP

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MM-CKcontentandcatalyticactivityweredecreasedduring.ppt

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MM-CKcontentandcatalyticactivityweredecreasedduring.ppt

MM-CK content and catalytic activity were decreased during CHF isolated myofibrils from sham and CHF LV were analyzed for M-CK content by Western blotting following functional analyses. Michael J. Mihm et al. Cardiovasc Res 2001;49:798-807 Copyright ? 2001, European Society of Cardiology MM-CK content and catalytic activity were decreased during CHF isolated myofibrils from sham and CHF LV were analyzed for M-CK content by Western blotting following functional analyses. A Representative Western blot of pure M-CK dilutions probed with anti-M-CK antibody. Lanes 1–5: Dilutions of 5–0.32 μg M-CK. Lane 6: 0 μg M-CK. B Standard curve for M-CK immunoreactivity. M-CK signal is linearly related to M-CK content. C Representative Western blot of myofibrillar isolates from sham and CHF LV probed for M-CK. Lanes 1, 3, 5: sham control; Lanes 2, 4, 6: CHF. D Digital image analysis of M-CK Western blotting in CHF versus sham control. Despite decrease in content, significant impairments in the catalytic activity kcat of MM-CK were still observed. *, P 0.05.