性激素通过IL1_IL-1R通路促进原发性肝癌的机制的研究性激素通过IL-1_IL-1R通路促进原发性肝癌的机制的研究.docVIP

修改意见： 性激素通过IL-1/IL-1R通路促进原发性肝癌的机制的研究 [摘要]目的 探讨雌激素E2 Estradiol，E2 是否对原发性肝癌的发生发展有促进作用，以及该作用机制是否通过白细胞介素-1 Interleukin-1，IL-1 /白细胞介素-1受体（Interleukin-1 receptor，IL-1R）通路。方法 选取我院2013年1月至2014年6月接收的原发性肝癌男性患者作为观察组，选取同期接收的非原发性肝癌患者作为正常对照组，分别采用ELISA法检测两组患者血清中的E2、IL-1β和VEGF浓度，采用Western blot法检测两组肝肿瘤组织和正常肝组织中E2R和IL-1βR蛋白表达水平。结果 观察组患者血清中E2、IL-1β和血管内皮生长因子 Vascular Endothelial Growth Factor，VEGF 浓度显著性高于正常对照组，且两组间比较具有显著性差异（P 0.01）；观察组肿瘤组织中E2R和IL-1βR蛋白表达水平显著性高于正常对照组，且两组间比较具有显著性差。异（P 0.01）。结论 E2对原发性肝癌的发生发展具有促进作用，且改作用机制可能是通过上调IL-1/IL-1βR通路，促进VEGF的表达，引起肿瘤血管的形成而完成的。 [关键词] 雌激素；白细胞介素-1；白细胞介素-1受体；原发性肝癌 [中图分类号] TQ460. 7 [文献标识码]A Mechanism of primary liver cancer promoting hormone pathway through IL-1/IL-1R [Abstract] Objective To investigate whether estrogen E2 Estradiol, E2 for the development of primary liver cancer are promoted, as well as the mechanism of action is through interleukin -1 Interleukin-1, IL-1 / IL 1 receptor Interleukin-1 receptor, IL-1R pathway. Methods Selected male patients with primary liver cancer as the observation group of our hospital from January 2013 to June 2014. Selected the same period of non-primary liver cancer patients as normal control group, the patients were detected by ELISA serum E2, IL-1β and VEGF concentrations were detected by Western blot in liver tumor tissue and normal liver tissue and IL-1βR E2R protein levels. Results Serum observation group E2, IL-1β, and vascular endothelial growth factor Vascular Endothelial Growth Factor, VEGF were significantly higher than the normal control group, and between the two groups was significant difference P 0.01 ; Observer Group E2R tumor tissue and IL-1βR protein levels were significantly higher than the normal control group, and between the two groups was significant difference P 0.01 . Conclusion The development of E2 to promote the role of primary liver cancer, and the mechanism of action may be changed by upregulating IL-1/IL-1βR pathway promotes the expression of VEGF, causing the forma