草鱼烂尾病病原细菌Y2的分离鉴定与中西药物敏感性解决方案.doc

武汉轻工大学 毕业论文 2013届 毕业论文题目：草鱼烂尾病病原细菌Y2的分离鉴定与中西药物敏感性分析 姓 名 蔡庐山 学 号 090702228 院（系） 动物科学与营养工程学院 专 业 水产养殖学 年 级 2009级 指导教师 刘洪明 武汉轻工大学制 2013 年 05 月 目 录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc264151151 摘 要 1 HYPERLINK \l _Toc264151152 1、前言 2 HYPERLINK \l _Toc264151153 2、材料与方法 9 HYPERLINK \l _Toc264151154 2.1细菌分离 9 HYPERLINK \l _Toc264151155 2.2病原菌的生理生化鉴定 9 HYPERLINK \l _Toc264151156 2.3待测细菌WH125的分子生物学鉴定 11 HYPERLINK \l _Toc264151157 2.4 细菌的药物敏感性测定 12 HYPERLINK \l _Toc264151160 3、结果 13 HYPERLINK \l _Toc264151161 3.1病鱼症状 13 HYPERLINK \l _Toc264151162 3.2病原菌的生理生化鉴定 13 HYPERLINK \l _Toc264151163 3.3分子生物学鉴定结果 16 HYPERLINK \l _Toc264151164 3.4菌种判定 18 HYPERLINK \l _Toc264151165 3.5药敏试验结果 18 HYPERLINK \l _Toc264151167 4、讨论 19 HYPERLINK \l _Toc264151168 参考文献 22 PAGE 8 草鱼烂尾病病原细菌Y2的分离鉴定 摘要 本文从实验室患病草鱼身上分离得到一株致病菌，通过对细菌分离培养与纯培养、形态与菌落特征、理化特性等表观分类学指征鉴定的测定；同时择代表菌株进行了16S rRNA基因的分子鉴定，测定了16S rRNA基因序列、分析了相关细菌相应序列的同源性、构建了系统发生树；以表型性状鉴定结果，及细菌发育学分析的结果，进行分离菌的种属判定。结果表明所检鱼病的致病菌为草鱼烂尾病病原细菌Y2。同时还对该病菌进行了药物敏感性测定，明确了该菌对各种中西药的敏感性，为治疗由草鱼烂尾病病原细菌Y2所引起的水产动物疾病提供了理论基础。 关键词：草鱼烂尾病病原细菌Y2 16S rRNA 药物敏感性 Isolation and identification of pathogenic bacteria Y2 of Grass carp tail rot Abstract In this paper, a pathogenic bacteria strains were isolated from laboratory sick grass carp. Cultivation of bacteria and pure culture, morphology and colony characteristics, physical and chemical properties such as apparent taxonomical indications identified measurement. In addition, molecular identification of analysis of the nucleotide sequence of the 16S rRNA gene were applied, the 16S rRNA gene were sequenced and the phylogenetic tree representing genetic relatedness among the isolates and publicly available 16S rRNA gene sequence from GenBank database was constructed; the results showed that the isolates were Aeromonas veronii. The Antimicrobial susceptibilities of selected strains to antimicrobial agents were determined, Results