RNA干扰技术技术总结.pptVIP

RNAi的应用 MicroRNA (miRNA): A New Class of Small RNA Molecules siRNA—小干扰RNA stRNA 小时序RNA miRNA microRNA It appears that “ tiny RNA world” may not be so tiny after all The DNA vector-based RNA interference (RNAi) technology BD? Knockout Adenoviral RNAi Systems T T T T T U6 Pro Sense Anti Sense Spacer Kan R pUC ori User defined sequence for RNAi pSIREN-Shuttle I-Ceu I PI-SceI BD? Knockout Adenoviral RNAi Systems 系统能快速、方便的形成表达发夹结构的siRNA的重组腺病毒，从而有效的侵染到各种宿主细胞。 BD Knockout Adenoviral RNAi System 1使用RNAi-ready pSIREN Shuttle vector，通过酶切连接构建腺病毒DNA质粒。可感染分裂及非分裂细胞，宿主细胞范围广，可实现高水平表达。能感染难以转染的细胞。 siRNA表达