miR-21如何促进毛囊神经嵴干细胞向许旺细胞分化.docVIP

、 miR-21 promotes the differentiation of hair follicle-derived neural crest stem cells into Schwann cells Yuxin Ni1, Kaizhi Zhang2, Xuejuan Liu3, Tingting Yang?1, Baixiang Wang?1, Li Fu?1, Lan A?1, Yanmin Zhou?1 1 Hospital of Stomatology, Jilin University, Changchun, Jilin Province, China2 China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China3 First Hospital of Jilin University, Changchun, Jilin Province, China 摘要? 毛囊神经嵴干细胞在体内外实验中均可分化为许旺细胞，但分化过程中的调节机制目前尚未完全清楚。实验从人毛囊中分离神经嵴干细胞转染miR-21后，定向诱导为许旺细胞。定量RT-PCR检测发现，miR-21在神经嵴干细胞分化为许旺细胞的过程中表达逐渐升高。转染miR-21激动剂agomir-21后，干细胞分化为许旺细胞的能力增强，而转染miR-21的抑制剂antagomir-21后，干细胞的分化能力则减弱。进一步研究发现，SOX2是miR-21的作用靶点，miR-21可在不影响SOX2 mRNA水平的情况下通过与其3’-UTR段结合而下调SOX蛋白表达。结果证实，在神经嵴干细胞中敲除SOX2基因可显著逆转antagomir-21抑制干细胞分化为许旺细胞的能力，说明miR-21可通过与SOX2 mRNA 3’-UTR段结合下调其蛋白表达，从而促进神经嵴干细胞向许旺细胞分化，发挥修复周围神经损伤的作用。 关键词：?神经再生；?微小RNA；?干细胞；?许旺细胞；?SOX2；?毛囊；?神经嵴干细胞；?神经元；?NFSC grant； Fund:?国家自然科学基金项目（座机电话号码） Acceptance date:?2014-03-08 Cite this article: Yuxin Ni, Kaizhi Zhang, Xuejuan Liu, Tingting Yang, Baixiang Wang, Li Fu, Lan A, Yanmin Zhou. miR-21 promotes the differentiation of hair follicle-derived neural crest stem cells into Schwann cells[J].?Neural Regeneration Research, 2014, 9 8 : 828-836.