Antisensesuppressionofreplicasegeneexpressionrecover.pptVIP

Antisense suppression of replicase gene expression recoverstomato plants from leaf curl virus infection Shelly Praveena,*, Anil K. Mishraa, Arupratan Dasguptab Plant Science 168 (2005) 1011–1014 學生:林怡芳 Introduction 作者要把這ANTISENE送進被ToLCV感染的蕃茄, 使ToLCV病毒Rep gene silence  Methods Methods Analysis of transgenic plants PCR analysis PCR-Southern Discussion ToLCV-infected tomato plants containing antisense Rep gene construct 作者以他設計的實驗證明其Rep of ToLCV antisense gene 會使ToLCV病毒Rep gene silence * 7 days — young leaves start to curl 21 days — leaves curling and early stages of interveina