色谱双语教案分析.ppt

酶底物，激素-受体 * ：蛋白质表面一般有疏水与亲水集团，疏水层析是利用蛋白质表面某一部分具有疏水性，与带有疏水性的载体在高盐浓度时结合。在洗脱时，将盐浓度逐渐降低，因其疏水性不同而逐个地先后被洗脱而纯化，可用于分离其它方法不易纯化的蛋白质。 * Ion Exchange chromatography IEX Third step --- elution Ion Exchange chromatography IEX Third step --- elution Ion Exchange chromatography IEX Final step --- washing Ion Exchange chromatography IEX Re- -equilibration Use some very low ionic buffer usually use Deionized water to regenerate the media Gel filtration/Size exclusion Gel filtration separates molecules according to differences in size as they pass through a gel filtration medium packed in a column. U